| 伊氏锥虫细胞工程苗的研究 |
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| 引用本文： | 李国清,汪志楷.伊氏锥虫细胞工程苗的研究[J].中国农业大学学报,1995(Z1):43-46. |
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| 作者姓名： | 李国清 汪志楷 |
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| 作者单位： | 北京农业大学动物医学院; |
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| 基金项目： | 国家教委博士点基金资助 |
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| 摘 要： | 试验采用体外培养技术,通过接受γ-射线和紫外线辐照的影响,定向选育突变虫株。首次对伊氏锥虫的细胞工程苗进行了研究。试验结果表明受γ-射线或紫外线辐照的虫体接种小白鼠后,潜伏期和病程与对照组相比差异极显著(P<0.01)。辐照虫体体外培养后接种小白鼠,潜隐期和病程均与对照组相似。经紫外线处理后辐照的伊氏锥虫转入用小鼠腹腔巨噬细胞作支持细胞的培养基内培养,培养7～10d使突变表型表达。然后加入嘌呤类似物继续培养2周,筛选出基因突变株,建立细胞系。小白鼠试验测得该细胞系LD_(50)为10~(-3.5)·mL~(-1),比强毒株毒力减少60倍,其免疫保护率达90%。
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| 关 键 词： | 伊氏锥虫 细胞工程苗 辐照 |
Studies on Cellular Engineering Vaccine of Trypanosoma evansi |
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| Li Guoqing Wang Zhikai.Studies on Cellular Engineering Vaccine of Trypanosoma evansi[J].Journal of China Agricultural University,1995(Z1):43-46. |
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| Authors: | Li Guoqing Wang Zhikai |
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| Institution: | Li Guoqing Wang Zhikai (College of Veterinary Medicine,BAU,Beijing 100094) |
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| Abstract: | The cell system of T.evansi mutant was set up by means of directional cultivation of the parasite attenuated by irradiation,and its virulence and immunogenicity was determined,After mice were vaccinated with trypanosomes irradiated by gamma ray or ultraviolet ray,the difference between irradia- tion group and control group was significantly obvious in prepatent period and period of disease (P<0.01).While mice vaccinated with culture-derived trypanosomes after radiation were similar to con- trol in the prepa... |
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| Keywords: | Trypanosoma evansi cellular engineering vaccine irradiation |
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