| 木霉菌对番茄灰霉病菌的抑制作用 |
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| 引用本文： | 甘林,代玉立,杨秀娟,石妞妞,阮宏椿,杜宜新,陈福如.木霉菌对番茄灰霉病菌的抑制作用[J].福建农业学报,2016(11):1221-1225. |
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| 作者姓名： | 甘林 代玉立 杨秀娟 石妞妞 阮宏椿 杜宜新 陈福如 |
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| 作者单位： | 福建省农业科学院植物保护研究所，福建 福州 350013; 福建省作物有害生物监测与治理重点实验室，福建 福州 350003 |
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| 基金项目： | 福建省农业科学院青年基金项目(2014CX-16) |
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| 摘 要： | 为明确木霉菌对福建省番茄灰霉病菌的抑制效果,研究了8株木霉菌对病原菌的拮抗作用。结果表明,在对峙培养条件下,供试木霉菌能显著抑制灰霉病菌菌丝生长,其抑制率为86.83%~91.39%,不同木霉菌株的发酵液对病原菌菌丝生长具有不同程度的抑制效果,其差异达显著水平。其中,菌株FJ2006-8发酵液的抑制生长率最高,为39.44%。此外,供试的木霉菌均能产生非挥发性和挥发性代谢产物,其中,菌株FJ2006-8的非挥发性代谢产物对病原菌菌丝生长的抑制效果最佳,抑制率为61.09%;而菌株T05-58的挥发性代谢产物对病原菌菌丝生长的抑制率最高,为22.95%。研究结果可为木霉菌生防菌剂的开发以及番茄灰霉病的绿色防控提供理论依据。
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| 关 键 词： | 木霉菌 番茄灰霉病菌 拮抗作用 代谢产物 |
Antagonistic Effect of Trichoderma spp.Strains on Botrytis cinerea |
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| Abstract: | Antifungal activities of 8 strains of Trichoderma spp.on Botrytis cinerea in Fujian was studied.By means of confrontation culture,all of the strains were shown to effectively inhibit the mycelial growth of the pathogen with inhibition rates ranging from 86.83% to 91.39%.The fermentation broths of the Trichoderma strains differed significantly in their inhibiting effects on hyphal growth of the pathogen.The broth from FJ2006-8 was found to render the highest inhibition rate of 39.44% among all.All tested strains produced volatile as well as non-volatile metabolites.The non-volatile metabolites produced by FJ2006-8 showed the greatest inhibitory effect,reaching a rate of 61.09%,while the volatiles from T05-58 exhibited the highest rate of 22.95%.The results would provide the needed information to develop a biocontrol agent from Trichoderma for the disease on tomato plants caused by the gray mold. |
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| Keywords: | Trichoderma spp Botrytis cinerea antagonism metabolite |
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