| 高效液相色谱法测定鳗鲡各组织中的乙酰甲喹残留量 |
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| 引用本文： | 潘葳.高效液相色谱法测定鳗鲡各组织中的乙酰甲喹残留量[J].福建农业学报,2016(8):853-857. |
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| 作者姓名： | 潘葳 |
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| 作者单位： | 福建省农业科学院农业质量标准与检测技术研究所/福建省精密仪器农业测试重点实验室,福建 福州,350003 |
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| 基金项目： | 福建省科技计划项目---省属公益类科研院所基本科研专项(2014R1025-3) |
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| 摘 要： | 建立鳗鲡各组织中乙酰甲喹残留量的高效液相色谱测定方法,高效液相色谱检测条件为:采用SunFireTM C_(18)柱(4.6mm×150mm,3.5μm),柱温35℃;以甲醇∶水(40∶60,体积比)为流动相,流速为0.8mL·min~(-1);采用二极管阵列检测器,检测波长239nm。结果表明:在0.05~2.5μg·mL~(-1)范围内,乙酰甲喹具有良好的线性关系,含量与峰面积的相关系数达0.999以上;乙酰甲喹在鳗鲡肌肉、肾、肝和血浆中的检出限分别为3μg·kg~(-1)、15μg·kg~(-1)、15μg·kg~(-1)、15μg·L~(-1),鳗鲡各组织中乙酰甲喹回收率在76%~90%,相对标准偏差均小于10%(n=6)。本方法简便、快捷、灵敏度高,具有良好的重复性,满足鳗鲡各组织基质内乙酰甲喹残留量的快速定量分析的要求。
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| 关 键 词： | 乙酰甲喹 鳗鲡 高效液相色谱 肌肉 肝 肾 血浆 |
HPLC Analysis on Mequindox Residues in Tissues of Eel |
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| Abstract: | An HPLC methodology was developed for determining mequindox residues in the organs of eels.The analysis was conducted under the following conditions:SunFireTM C18 column (4.6 mm×150 mm,3.5 μm)at 35℃,methanol:deionized water (v/v 40∶60)for themobile phase with a flow rate of 0.8 mL·min-1 ,and 239 nm wavelength seton PDA detector for optical density measurements.With in the mequindox concentration range of 0.05-2.5μg·mL-1 ,an excellent linear regression between the drug concentration and the peak area under curve was obtained with a correlation coefficient greater than 0.9 9 9.LODs of mequindox in the muscle,liver,kidney,and plasma from the eelswere 3 μg · kg-1 ,1 5μg · kg-1 ,1 5 μg · kg-1 ,and 1 5 μg · L-1 ,respectively.The average mequindox recovery rate of the method ranged from 76% to 90% with RSD (n=6)below 10%.It was,thus, concluded that the newly developed simple,rapidmethodology was highly sensitive and repeatable in detecting mequindox residuein the tissues of the eels. |
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| Keywords: | mequindox eel HPLC muscle liver kidney plasma |
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