| 促生细菌Bacillus sp. QN2MO-1的鉴定及对番茄生长的影响 |
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| 引用本文： | 张妙宜,高祝芬,唐文,赵炎坤,李凯,王尉,谢江辉,张锡炎,黄绵佳.促生细菌Bacillus sp. QN2MO-1的鉴定及对番茄生长的影响[J].热带作物学报,2020,41(12):2474-2481. |
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| 作者姓名： | 张妙宜 高祝芬 唐文 赵炎坤 李凯 王尉 谢江辉 张锡炎 黄绵佳 |
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| 作者单位： | 1.海南大学园艺学院,海南海口 5702282.中国热带农业科学院热带生物技术研究所,海南海口 571101 |
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| 基金项目： | 国家重点研发计划项目(2017YFD0202105);现代农业产业技术体系建设专项资金项目(CARS 31) |
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| 摘 要： | 为分离和鉴定具有促生作用的内生菌,开发潜在功能的微生物菌株,本研究以诺尼（Morinda citrifolia L.）为研究对象,从植株不同组织中共分离得到621株内生细菌,经复筛得到具有促生和解磷作用的菌株,命名为QN2MO-1。基于菌株的生理生化分析和16S rDNA的序列比对,该内生细菌与Bacillus siamensis KCTC 13613T（AJVF01000043）具有99.04%的同源性。药敏性分析显示,该菌对青霉素、硫酸链霉素和氨苄西林具有明显的抗性。QN2MO-1可促进番茄种子的萌发;与对照相比,5%菌体发酵液处理能显著提高植株的生长。
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| 关 键 词： | 诺尼 暹罗芽胞杆菌 促生 种子萌发 田间试验 |
| 收稿时间： | 2019-12-11 |
Identification of Probiotic Bacterium Bacillus sp. QN2MO-1 and Its Effect on Tomato Growth |
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| ZHANG Miaoyi,GAO Zhufen,TANG Wen,ZHAO Yankun,LI Kai,WANG Wei,XIE Jianghui,ZHANG Xiyan,HUANG Mianjia.Identification of Probiotic Bacterium Bacillus sp. QN2MO-1 and Its Effect on Tomato Growth[J].Chinese Journal of Tropical Crops,2020,41(12):2474-2481. |
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| Authors: | ZHANG Miaoyi GAO Zhufen TANG Wen ZHAO Yankun LI Kai WANG Wei XIE Jianghui ZHANG Xiyan HUANG Mianjia |
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| Institution: | 1. College of Horticulture, Hainan University, Haikou, Hainan 570228, China2. Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China |
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| Abstract: | To identify endophytic bacteria with growth-promoting function and develop potential functional microbial strains, we isolated 621 strains from different tissues of Noni (Morinda citrifolia L.). After screening, a strain with growth-promoting and phosphate-solubilizing function was obtained and named QN2MO-1. Based on the physiological and biochemical analysis of the strain and the sequence alignment of 16S rDNA, the endophyte had 99.04% homology with Bacillus siamensis KCTC 13613T (AJVF01000043). Antibiotic sensitivity analysis showed that the strain had obvious resistance to penicillin, streptomycin sulfate and ampicillin. Compared with the control, QN2MO-1 could promote the germination of tomato seeds and 5% of fermentation broth treatment could significantly improve plant growth. |
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| Keywords: | Morinda citrifolia L Bacillus siamensis growth-promoting seed germination field trial |
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