| 华石斛组培苗炼苗基质的筛选与优化 |
| |
| 引用本文： | 姚肖健,宋希强,杨福孙.华石斛组培苗炼苗基质的筛选与优化[J].热带作物学报,2020,41(11):2226-2231. |
| |
| 作者姓名： | 姚肖健 宋希强 杨福孙 |
| |
| 作者单位： | 海南大学热带作物学院/海南大学热带特色林木花卉遗传与种质创新教育部重点实验室,海南海口 570228 |
| |
| 基金项目： | 海南省创新研究团队项目(2018CXTD334);国家自然科学基金项目(31160399);中央财政林业科技推广示范资金项目(琼[2015]TG03号) |
| |
| 摘 要： | 基质通过影响水肥吸收和根系通气度,进而影响石斛组培苗的生长,对石斛组培苗的人工驯化栽培具有至关重要的作用。本研究以华石斛组培苗为对象,采用4种基质配比对组培苗进行炼苗试验,通过测定组培苗的存活率、形态指标、壮苗指数和光合指标,筛选出适宜华石斛组培苗炼苗的基质。结果表明：以5 cm透明塑料杯装16.3 g水苔,其孔隙度和气水比均较高,达42.1%和2.51;保水、通气性好,利于华石斛组培苗生根、长芽,组培苗存活率较高,达56.3%,壮苗指数高达0.016;叶绿素相对含量和净光合速率最高;105孔穴盘,每孔6.0 g水苔,孔隙度和气水比低,基质量少,组培苗存活率低。表明以5 cm透明塑料杯装16.3 g水苔可作为华石斛大棚炼苗的优良基质。
|
| 关 键 词： | 华石斛 组培苗 驯化 栽培基质 光合生理 |
| 收稿时间： | 2019-10-18 |
Selection and Optimization of Matrix for Seedlings of Dendrobium sinense in vitro |
| |
| YAO Xiaojian,SONG Xiqiang,YANG Fusun.Selection and Optimization of Matrix for Seedlings of Dendrobium sinense in vitro[J].Chinese Journal of Tropical Crops,2020,41(11):2226-2231. |
| |
| Authors: | YAO Xiaojian SONG Xiqiang YANG Fusun |
| |
| Institution: | College of Tropical Crops, Hainan University / Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Hainan University, Haikou, Hainan 570228, China |
| |
| Abstract: | Substrate plays an important role in the artificial domestication and cultivation of Dendrobium sinense seedlings by affecting the water and fertilizer absorption and root aeration, and then affecting the growth of D. sinense seedlings. In this experiment, the tissue culture plantlet of D. sinense was used as the research object, and four media were used to test the plantlet. By measuring the survival rate, morphological index, strong seedling index and photosynthetic index of the tissue culture plantlet, the suitable media was selected. The results showed that the porosity and air-water ratio of 16.3 g water moss packed in 5 cm transparent plastic cup were higher, which was 42.1% and 2.51; the water conservation and aeration were good, which were beneficial to the rooting and sprouting of the tissue cultured seedlings of D. sinense. The survival rate of the tissue cultured seedlings was higher, which was 56.3%, and the strong seedling index was 0.016; the relative chlorophyll content and net photosynthetic rate were the highest; the porosity and air-water ratio of 6.0 g water moss per hole in the 105 hole disc were relatively low, and the survival rate of plantlets was low because of less matrix. Therefore, 16.3 g water moss in a 5 cm transparent plastic cup can be used as a better substrate for the cultivation of D. sinense. |
| |
| Keywords: | Dendrobium sinense tissue culture seedling acclimation cultivated matrix photosynthetic physiology |
|
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
|
