| 一株临沧野生灵芝的鉴定与驯化栽培 |
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| 引用本文： | 张焱珍,周会明,白玉英,柴红梅,古广红,赵娅通,田利波,卯明娟.一株临沧野生灵芝的鉴定与驯化栽培[J].热带作物学报,2020,41(11):2176-2182. |
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| 作者姓名： | 张焱珍 周会明 白玉英 柴红梅 古广红 赵娅通 田利波 卯明娟 |
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| 作者单位： | 1.滇西科技师范学院生物技术与工程学院,云南临沧 6770002.云南省农业科学院生物技术与种质资源研究所,云南昆明 650221 |
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| 基金项目： | 云南省教育厅科学研究基金项目(2019J0491);国家食用菌产业技术体系项目(CARS-20) |
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| 摘 要： | 从采集于云南临沧的野生灵芝子实体中分离纯化获得纯培养物,编号为YAASM4672,结合形态学与ITS序列分析鉴定其分类学地位,并设计了9种栽培种培养基,测定其栽培特性。结果显示,采集到的菌株YAASM4672为灵芝（Ganoderma lingzhi）,其形态特征与分子鉴定结果一致。该菌在2号栽培种配方上,菌丝生长和子实体农艺性状（覆土组优于露地组）表现最好。菌丝体白色,长势浓密,38 d满袋,平均生长速度达(5.89±1.01) mm/d;菌盖黄色,直径达66.00 mm,平均生长速度最快为(4.14±1.07) mm/d,与其他配方相比,达到显著（P<0.05）或极显著差异（P<0.01）;菌柄褐黄,其长度、直径分别达到84.00 mm、18.00 mm。驯化的野生灵芝菌株YAASM4672人工栽培最优配方为：78%玉米芯、18%米糠、2%高粱粉、1%石膏粉、1%白砂糖,覆土能促进其子实体的生长发育。
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| 关 键 词： | 灵芝 形态学 ITS序列分析 驯化栽培 |
| 收稿时间： | 2019-11-28 |
Identification and Domestication Cultivation of a Wild-type Ganoderma lingzhi Strain from Lincang,Yunnan |
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| ZHANG Yanzhen,ZHOU Huiming,BAI Yuying,CHAI Hongmei,GU Guanghong,ZHAO Yatong,TIAN Libo,MAO Mingjuan.Identification and Domestication Cultivation of a Wild-type Ganoderma lingzhi Strain from Lincang,Yunnan[J].Chinese Journal of Tropical Crops,2020,41(11):2176-2182. |
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| Authors: | ZHANG Yanzhen ZHOU Huiming BAI Yuying CHAI Hongmei GU Guanghong ZHAO Yatong TIAN Libo MAO Mingjuan |
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| Institution: | 1. School of Biotechnology and Engineering, West Yunnan University, Lincang, Yunnan 677000, China2. Institute of Biotechnology and Germplasmic Resources, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650221, China |
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| Abstract: | An optimal medium was screened for the cultivation of a wild-type Ganoderma lingzhi, which are the basis of its mass production. In this paper, a strain of YAASM4672 was isolated from a wild mushroom fruiting body of Ganoderma lingzhi, from Lincang, Yunnan. Taxonomic status was identified based on its morphology and ITS sequence analysis, and nine media of culture spawn were designed for the determination of its cultivation characteristics. The result showed that the wild strain of YAASM4672 was G. lingzhi, and the morphological characteristics were consistent with its molecular identification. Formula 2 of culture spawn were the best for the mycelial growth and agronomic characters of fruiting body (soil mulched group was better than the open field group) of strain YAASM4672. Its mycelia colour was white and dense, the time of sackful was 38 d, and the growth rate of mycelia was (5.89±1.01) mm/d. The pileus was yellow, diameter was 66.00 mm, and average growth rate was (4.14±1.07) mm/d, which were significantly (P<0.05) or extremely significantly (P<0.01) different compared with other formula. Stipe was brownish yellow, its length and diameter was 84.00 mm and 18.00 mm respectively. In short, the optimal medium for artificial cultivation of strain YAASM4672 was 78% corn cob, 18% rice bran, 2% sorghum flour, 1% gypsum powder, and 1% white granulated sugar. Soil mulched can promote the growth and development of its fruiting body. |
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| Keywords: | Ganoderma lingzhi morphology ITS sequencing analysis domestication cultivation |
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