| 根袋法获取木薯根际土壤及其细菌群落特征研究 |
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| 引用本文： | 韦云东,罗燕春,郑华,李军,盘欢,雷开文,徐钏.根袋法获取木薯根际土壤及其细菌群落特征研究[J].热带作物学报,2020,41(9):1928-1938. |
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| 作者姓名： | 韦云东 罗燕春 郑华 李军 盘欢 雷开文 徐钏 |
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| 作者单位： | 1.广西壮族自治区亚热带作物研究所,广西南宁 5300012.广西壮族自治区木薯研究所,广西南宁 530001 |
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| 基金项目： | “食用糯性木薯新品种‘桂热9号’的中试和开发利用”项目(桂科AB18221046);现代农业产业技术体系建设专项资金项目(CARS-11);“基于水肥一体化的木薯水肥耦合效应研究”项目(桂热研201806) |
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| 摘 要： | 为了获取木薯根际土壤并研究其细菌群落特征,加深对木薯根际微生态的认知,开展根袋试验,以粘土和砂质壤土根袋内不同土壤质量（100~500 g）为处理。结果表明,根袋内土质量影响株高,但不影响茎叶重。根袋内100 g土的速效养分受到根系强烈影响,其中铵态氮和速效钾显著高于根袋外。Illumina Hiseq高通量测序结果表明,细菌优势菌门包括变形菌门（31.272%）、放线菌门（25.753%）、酸杆菌门（12.761%）、绿弯菌门（8.799%）等。2种土壤根袋内细菌α多样性增加,粘土细菌丰度也增加。RDA分析表明,土壤速效磷与放线菌门和绿弯菌门正相关,与变形菌门负相关;速效氮、速效钾、铵态氮、硝态氮与芽单胞菌门、酸杆菌门、Parcubacteria等负相关,与厚壁菌门、蓝藻门、梭杆菌门、拟杆菌门正相关;从根袋内外土壤速效养分和土壤细菌多样性综合判定,根袋内100 g土壤时可视为根际土壤,且铵态氮和速效钾可作为判断是否根际土壤的指示指标。根际微生物多样性显著提高。鞘脂杆菌目是木薯品种SC205在不同土质下共同富集的根际细菌。发现了土壤速效养分与部分细菌门的相关性。
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| 关 键 词： | 木薯根际 铵态氮 速效钾 微生物群落 鞘脂杆菌目 |
| 收稿时间： | 2019-10-30 |
Cassava Rhizosphere Soil Collected by “Root Bag” Method and Its Bacteria Diversity |
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| WEI Yundong,LUO Yanchun,ZHENG Hua,LI Jun,PAN Huan,LEI Kaiwen,XU Chuan.Cassava Rhizosphere Soil Collected by “Root Bag” Method and Its Bacteria Diversity[J].Chinese Journal of Tropical Crops,2020,41(9):1928-1938. |
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| Authors: | WEI Yundong LUO Yanchun ZHENG Hua LI Jun PAN Huan LEI Kaiwen XU Chuan |
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| Institution: | 1. Guangxi Institute of Subtropical Crops, Nanning, Guangxi 530001, China2. Guangxi Institute of Cassava, Nanning, Guangxi 530001, China |
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| Abstract: | In order to collect cassava rhizosphere soil and study its bacteria community, and further deepen the knowledge of the micro-ecology of the rhizosphere soil, a field trial with the “root bag” method was carried out with two soil textures (clay and sandy roam) and a series of soil quantity from 100g to 500g in the root bag. Cassava plant height was significantly influenced by the soil quantity in the root bag, while cassava shoot weight was not. Soil available nutrients were strongly affected by roots when soil quantity in the root bag was only 100g, ammonium content (NH4-N) and available potassium (AK) was enriched significantly compared to bulk soil. Results from high throughout sequencing by Illumina Hiseq showed that the dominant bacterial community was Proteobacteria (31.272%), Actinobacteria (25.753%), Acidobacteria (12.761%), Chloroflexi (8.799%) et al. Results from α diversity indexes showed that the diversity of bacteria was increased in the rhizospheric soil, and the species richness was also increased in the clay. Compared to bulk soil, the soil samples collected from the inside “root bag” was relatively rich in Sphingobacteriales, Rhizobiales, Xanthomonadales, Verrucomicrobia and related phylum, class, genus and species for the clay, and for the sandy roam, rich in Sphingobacteriales and genus and species in gemmatimonadaceae. Results from Redundancy analysis (RDA) triplots demonstrated that soil available phosphorus (AP) was positively correlated to Actinobacteria and Chloroflexi, and negatively correlated to Proteobacteria; Soil available nitrogen (AN), AK, NH4-N, nitrates nitrogen (NO3-N) were negatively correlated with Gemmatimonadetes, Acidobacteria, Parcubacteria, Elusimicrobia, Armatimonadetes, TM6_Dependentiae, Chlorobi et al., and positively correlated with Firmicutes, Cyanobacteria, Fusobacteria, Bacteroidetes. In concusion, the 100g soil in the root bag could be seen as cassava rhizospheric soil according to soil nutrients and bacteria diversity. And NH4-N and AK could be regarded as the indicators as cassava rhizospheric soil. Soil microbial diversity was improved in the rhizosphere, and Sphingobacteriales was the enriched bacteria in both soil textures. Soil nutrients were correlated with some classification of soil bacterial. |
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| Keywords: | cassava rhizosphere ammonium nitrogen available phosphorus microbial community Sphingobacteriales |
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