| 香蕉枯萎病菌分子检测研究进展 |
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| 引用本文： | 杨迪,杜婵娟,叶云峰,彭军,张欣,付岗.香蕉枯萎病菌分子检测研究进展[J].热带作物学报,2020,41(12):2582-2590. |
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| 作者姓名： | 杨迪 杜婵娟 叶云峰 彭军 张欣 付岗 |
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| 作者单位： | 1.广西农业科学院植物保护研究所/广西作物病虫害生物学重点实验室,广西南宁 5300072.广西农业科学院园艺研究所,广西南宁 5300073.中国热带农业科学院环境与植物保护研究所,海南海口 571101 |
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| 基金项目： | 广西自然科学基金项目(2017GXNSFDA198002);广西创新驱动发展专项(桂科AA18118028-5);现代农业产业技术体系建设专项资金项目(CARS-31-07) |
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| 摘 要： | 香蕉枯萎病是全世界香蕉产业共同面临的毁灭性病害,但目前生产上仍缺乏适宜的抗病品种和有效的治疗措施。因此,借助快速准确的枯萎病菌检测技术及时明确病原菌以控制该病的传播和蔓延显得尤为重要。本文回顾了近年来国内外香蕉枯萎病菌分子检测技术的发展历程,归纳和总结了DNA指纹图谱、普通PCR、多重PCR、荧光定量PCR及等温扩增技术在该病菌检测中的研究进展,分析了不同检测技术的优缺点,并指出可能存在的问题和研究发展方向,为该病的分子检测技术优化和防控策略制定提供参考。
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| 关 键 词： | 香蕉枯萎病 尖孢镰刀菌古巴专化型 分子检测 |
| 收稿时间： | 2020-07-08 |
Research Advances on Molecular Detection of Banana Fusarium Wilt |
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| YANG Di,DU Chanjuan,YE Yunfeng,PENG Jun,ZHANG Xin,FU Gang.Research Advances on Molecular Detection of Banana Fusarium Wilt[J].Chinese Journal of Tropical Crops,2020,41(12):2582-2590. |
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| Authors: | YANG Di DU Chanjuan YE Yunfeng PENG Jun ZHANG Xin FU Gang |
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| Institution: | 1. Institute of Plant Protection Research, Guangxi Academy of Agricultural Sciences / Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi 530007, China2. Institute of Horticulture, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China3. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China |
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| Abstract: | Banana Fusarium wilt is a devastating disease in global banana industry. At present, there is still no available measures can be used for curing this disease, and lack of suitable resistant varieties. So, it is very important that developing the rapid and accurate detection technology in order to slow down the spread speed of banana Fusarium wilt in the field. In this paper, the development of molecular detection techniques on Fusarium oxysporum f. sp. cubense was reviewed. The detection technology based on DNA fingerprinting, general PCR, multiplex PCR, fluorescence quantitative PCR and isothermal amplification were summarized. The advantages and disadvantages of different detection technologies were analyzed. Futhermore, the problems lied in detection of this pathogen and possible research directions in the future were pointed out. |
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| Keywords: | banana Fusarium wilt Fusarium oxysporum f sp cubense molecular detection |
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