| 山西省主推玉米杂交种纯度鉴定SSR核心引物的筛选 |
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| 引用本文： | 王陆军,白建荣,王艳梅,王燕,赵文博,苏亮.山西省主推玉米杂交种纯度鉴定SSR核心引物的筛选[J].山西农业科学,2010,38(1):19-22. |
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| 作者姓名： | 王陆军 白建荣 王艳梅 王燕 赵文博 苏亮 |
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| 作者单位： | 1. 山西省农业科学院作物遗传研究所,山西,太原,030031
2. 山西大学生物工程中心,山西,太原,030006 |
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| 基金项目： | 山西省科技攻关项目，山西省科技基础条件平台建设项目 |
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| 摘 要： | 选用10对SSR核心引物对18份山西省玉米杂交种进行DNA指纹分析,并综合考虑多态性和杂合率,认为umc1590和umc1196这2对引物可鉴定17个品种纯度,phi402893,phi102228,phi233376,p-phi072,umc1066和phi022也可作为纯度鉴定的引物。建议采用umc1590,phi402893,phi233376,phi022这4对引物对18个杂交种纯度进行检测。
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| 关 键 词： | 玉米 SSR 纯度鉴定 核心引物 |
Selection of SSR Core Primers for Maize Hybrid Purity Identification in Shanxi Province |
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| WANG Lu-jun,BAI Jian-rong,WANG Yan-mei,WANG Yan,ZHAO Wen-bo,SU Liang.Selection of SSR Core Primers for Maize Hybrid Purity Identification in Shanxi Province[J].Journal of Shanxi Agricultural Sciences,2010,38(1):19-22. |
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| Authors: | WANG Lu-jun BAI Jian-rong WANG Yan-mei WANG Yan ZHAO Wen-bo SU Liang |
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| Institution: | WANG Lu-jun1,BAI Jian-rong1,WANG Yan-mei1,WANG Yan2,ZHAO Wen-bo2,SU Liang2(1.Crop Genetics Institute,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China,2.Bioengineering Centre,Shanxi University,Taiyuan 030006,China) |
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| Abstract: | 18 maize hybrids were analyzed with 10 SSR core primers.According to the polymorphism and heterogosite rate,17 maize hybrids could be identified with umc1590 and umc1196,and phi402893,phi102228,phi233376,p-phi072,umc1066 and phi022 could also be thought as suitable primers for identifying maize hybrids.Four primers were suggested to identify 18 maize hybrids. |
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| Keywords: | SSR Maize SSR Purity identification Core primers |
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