| 灵芝与糙皮侧耳原生质体融合子生物学特性的初步研究 |
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| 引用本文： | 王淑珍,范俊,扬家峰,扬英杰,白晨.灵芝与糙皮侧耳原生质体融合子生物学特性的初步研究[J].食用菌学报,2003,10(2):5-8. |
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| 作者姓名： | 王淑珍 范俊 扬家峰 扬英杰 白晨 |
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| 作者单位： | 1. 上海师范大学生命与环境科学学院,上海,200234
2. 复旦大学生命科学学院,上海,200433 |
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| 摘 要： | 以获得弱木栓质化灵芝菌株为目的，利用液体培养4d的灵芝菌丝体和液体培养3d的糙皮侧耳菌丝体为材料，在PEG-Ca^2 系统中诱导原生质体融合，获得初筛融合子19株(F1～F19)。其中，融合子F8的菌丝生长速度和生物量均接近糙皮侧耳，显微镜下，其菌丝体与灵芝和糙皮侧耳的均不相同，木栓质化程度大于糙皮侧耳，小于灵芝。
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| 关 键 词： | 灵芝 糙皮侧耳 原生质体融合子 生物学特性 菌丝生长速度 生物量 木栓质化 |
| 文章编号： | 1005-9873(2003)02-0005-04 |
| 修稿时间： | 2002年11月26 |
A Preliminary Research on the Biological Characteristics of G.lucidum and P.ostreatus Protoplast Fusants |
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| WANG Shu zhen ,FAN Jun ,YANG Jia feng ,YANG Ying jie ,BAI Chen.A Preliminary Research on the Biological Characteristics of G.lucidum and P.ostreatus Protoplast Fusants[J].Acta Edulis Fungi,2003,10(2):5-8. |
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| Authors: | WANG Shu zhen FAN Jun YANG Jia feng YANG Ying jie BAI Chen |
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| Institution: | WANG Shu zhen 1,FAN Jun 1,YANG Jia feng 1,YANG Ying jie 1,BAI Chen 2 |
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| Abstract: | In order to obtain the lower corkification strain of Ganoderma lucidum, the mycelia of G.lucidum and Pleurotus ostreatus were put under submerged culture for 4d and 3d resp.to prepare the protoplasts. And the fusion experiment of G.lucidum and P.ostreatus protoplasts was carried out under PEG-Ca + system. Ninteen fusants numbered from F1 to F19 were selected. Among them, the mycelial growth rate and the mycelial biomass of F8 were more similar to that of P.ostreatus, and the mycelia of F8 were very different from that of G.lucidum and P.ostreatus under the microscope.Furthermore, the corkification level of F8 mycelia was between that of G.lucidum and P.ostreatus . |
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| Keywords: | Ganoderma lucidum Pleurotus ostreatus Fusant Biological characteristic |
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