| 马齿苋多糖对tau蛋白磷酸化影响的研究 |
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| 引用本文： | 康洁.马齿苋多糖对tau蛋白磷酸化影响的研究[J].中国农学通报,2010,26(14):113-116. |
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| 作者姓名： | 康洁 |
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| 作者单位： | 商丘师范学院生命科学系,河南商丘,476000 |
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| 基金项目： | 河南省科技厅基础与前沿研究"马齿苋多糖对神经细胞保护作用的研究" |
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| 摘 要： | 目的是研究马齿苋多糖对神经细胞骨架微管结合蛋白-tau蛋白的磷酸化影响。方法通过构建基因表达载体pEGFP- tau-s3和转染表达载体pEGFP- tau-s3到3T3细胞，创造体外神经细胞骨架研究模型；通过共转染pEGFP- tau-s3与pcDNA-GSK-3β到3T3细胞、荧光显微观察和Wstern blotting实验，证明了马齿苋多糖对tau蛋白的磷酸化有影响，可以抑制GSK激酶对tau蛋白的磷酸化。结论马齿苋多糖对神经细胞微管骨架有保护作用
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| 关 键 词： | 玉米 玉米 双列杂交 配合力 遗传参数 |
| 收稿时间： | 2009/3/16 0:00:00 |
| 修稿时间： | 2009/4/22 0:00:00 |
Effect of polysaccharide from Portulaca oleracea on tau protein phosphorylation |
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| Kang Jie.Effect of polysaccharide from Portulaca oleracea on tau protein phosphorylation[J].Chinese Agricultural Science Bulletin,2010,26(14):113-116. |
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| Authors: | Kang Jie |
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| Institution: | Kang Jie(Department of Biology Shangqiu Teachers College,Shangqiu Henan 476000 ) |
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| Abstract: | This experiment was conducted to investigate effect of polysaccharide from portulaca oleracea on neuronal cytoskeleton microtubule protein （Tau） phosphorylation.Model for neuronal cytoskeleton was established by construction of gene expression vector and transfection vehicle of pEGFP-Tau-s3 in 3T3 cell.Co-transfection of pEGFP-Tau-s3 and pcDNA-GSK-3β in 3T3 cell,fluorescence microscope observation,and western blotting were used in this study.Result showed that polysaccharide from Portulaca oleracea restrained effects of GSK kinase on Tau phosphorylation.These data suggest that Portulaca oleracea might have protection effect on neuronal cytoskeleton microtubule. |
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| Keywords: | polysaccharide from Portulaca oleracea neuronal cytoskeleton Tau protein GSK kinase phosphorylation |
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