| 放线菌菌株97发酵液中活性代谢产物的抑菌作用及稳定性测定 |
| |
| 引用本文： | 张茹萍,郑洪波,贾春燕,贾玉,郑翠梅,张广民.放线菌菌株97发酵液中活性代谢产物的抑菌作用及稳定性测定[J].中国农学通报,2010,26(18):279-283. |
| |
| 作者姓名： | 张茹萍 郑洪波 贾春燕 贾玉 郑翠梅 张广民 |
| |
| 作者单位： | 山东农业大学植物保护学院,山东泰安,271018 |
| |
| 基金项目： | 山东省烟草专卖局科技项目 |
| |
| 摘 要： | 本实验室从采自山东临沂和日照的烟田土样中分离得到一株对烟草黑胫病菌(Phytophthora parasitica var. nicotianae)具有拮抗作用的放线菌菌株97，为了明确该生防菌的抑菌作用和抑菌因子的理化性质，采用菌丝生长速率法，对菌株97发酵液中活性代谢产物的抑菌作用及稳定性进行了研究。结果表明，发酵液中活性代谢产物对多种植物病原真菌均表现较好的抑制作用。此外，发酵液中活性代谢产物在低温贮藏下仍有很好的抑菌效果，在偏酸性或中性条件下稳定，有较强的热稳定性和抗紫外线照射能力，对蛋白酶K稳定性较好。
|
| 关 键 词： | 条华蜗牛 条华蜗牛 翻身时间 体重 温度 光照 饥饿 取食 |
| 收稿时间： | 4/6/2010 12:00:00 AM |
| 修稿时间： | 2010/5/25 0:00:00 |
Study on Antimicrobial Activity and Stability of Bioactive Metabolite Produced by Actinomycete 97 Strain Fermentation |
| |
| Zhang Ruping,Zheng Hongbo,Jia Chunyan,Jia Yu,Zheng Cuimei,Zhang Guangmin.Study on Antimicrobial Activity and Stability of Bioactive Metabolite Produced by Actinomycete 97 Strain Fermentation[J].Chinese Agricultural Science Bulletin,2010,26(18):279-283. |
| |
| Authors: | Zhang Ruping Zheng Hongbo Jia Chunyan Jia Yu Zheng Cuimei Zhang Guangmin |
| |
| Institution: | (College of Plant Protection, Shandong Agriculture University, Tai′an Shandong 271018) |
| |
| Abstract: | A study on antifungal action to 14 fungal pathogens and stability of bioactive metabolite produced by Actinomycete strain 97 fermentation was launched by means of growth speed method for the sake of evaluating the antifungal action and physicochemical characteristics of inhibitive factors of strain 97 and determine whether or not the strain posesses high potential value of biological control. The results showed that the bioactive metabolite had almost 60% inhibition rate to 5 pathogenic fungi （35.7% of the total）, and exhibited antagonistic abilities to several other plant pathogens. In addition, it still had a high activity when stored up at 4℃, and was stable to acidic, neutral and heat, maintained the antifungal activity after treatment of proteinase K and ultraviolet radiation, which laid the theoretical foundation for further study of strain 97. |
| |
| Keywords: | Actinomycete 97 strain bioactive metabolite antifungal activity stability |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
|
