| 开菲尔中产类细菌素乳酸菌的分离鉴定 |
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| 引用本文： | 王志博,郭德军.开菲尔中产类细菌素乳酸菌的分离鉴定[J].农产品加工．学刊,2011(7):69-73. |
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| 作者姓名： | 王志博 郭德军 |
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| 作者单位： | 黑龙江八一农垦大学食品学院,黑龙江大庆,163319 |
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| 摘 要： | 通过牛津杯法,从250株分离自开菲尔颗粒的乳酸菌中筛选出1株有较好抑菌活性的菌株,通过对个体特征、生理生化试验、16SrDNA序列对比,确定此株菌为乳酸乳球菌,命名为L11。在排除有机酸和过氧化氢的干扰后,发酵上清液对金黄色葡萄球菌还有抑菌活性,该菌株经过胃蛋白酶、胰蛋白酶处理后,发酵液的抑菌活性没有明显变化,说明抑菌物质为非蛋白成分,属于类细菌素,它具有较好的热稳定性,对蛋白酶不敏感,且具有较广的抗菌谱。
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| 关 键 词： | 开菲尔 乳酸乳球菌 分离鉴定 类细菌素 |
Isolation and Identification of Lactic Acid Bacteria Producing Class Bacteriocins from Kefir Grains |
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| Wang Zhibo,Guo Dejun.Isolation and Identification of Lactic Acid Bacteria Producing Class Bacteriocins from Kefir Grains[J].Nongchanpin Jlagong.Xuekan,2011(7):69-73. |
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| Authors: | Wang Zhibo Guo Dejun |
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| Institution: | Wang Zhibo,Guo Dejun(College of Food Science,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China) |
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| Abstract: | The strain is one of the 250 strains which isolated in the lactic acid bacteria from Kefir grains by Oxford cup.The strain is identified as the Lactococcus lactis by individual characteristics,physiological and biochemical experiments,16SrDNA sequence alignment and named L11.After the exclusion of organic acids and hydrogen peroxide interference,the ferment supernatant also has antibacterial activity to Staphylococcus aureus.After the treatment of pepsin and trypsin,antimicrobial activity of fermentation do... |
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| Keywords: | kefir grain lactococcus lactics isolation and identification class bacteriocins |
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