| 家蚕scaffold中新微卫星标记的获得与Dll基因的遗传连锁分析 |
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| 引用本文： | 郭秋红,詹帅,相辉,赵云坡,李卫华,黄勇平.家蚕scaffold中新微卫星标记的获得与Dll基因的遗传连锁分析[J].蚕业科学,2007,33(2):187-194. |
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| 作者姓名： | 郭秋红 詹帅 相辉 赵云坡 李卫华 黄勇平 |
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| 作者单位： | 1. 中国科学院上海生命科学院植物生理生态研究所,上海,200032
2. 江苏科技大学生物与环境工程学院,镇江,212018 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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国家重点基础研究发展计划(973计划) |
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| 摘 要： | 在进行家蚕遗传连锁图谱的整合过程中,需要寻找两个作图群体中都有多态的共有标记,但这种共有标记数量较少。为此,利用已有的简单重复序列(SSR)与家蚕基因组进行比对,寻找到匹配的scaffold,再采用SS-RHunter1.3搜索其中的SSR区域,排除掉原有SSR序列,选择重复次数在6～23之间的微卫星区域设计引物,用BC1群体的亲本及F1进行多态性的筛选,选择有多态性的标记用7019×(F50B×7019)BC1群体进行基因型分析。结果显示:根据原家蚕第2连锁群上SSR位点新设计的邻近的7对引物中,有6对引物在BC1群体的亲本中有多态性,选择其中2个进行遗传连锁分析,作图结果与原有相应SSR标记的作图结果基本保持一致,其中根据S0207所在scaffold上开发的NS02071和NS02072之间的图距达到6.9 cM;根据家蚕大造和C108的回交一代初步定位的D ll基因的位置与后来在其所在scaffold上所设计的临近引物D ll1和D ll2的定位一致,且这两个标记在遗传连锁图上的图距为0.0 cM,表明这两个标记在遗传图上的位置重叠。由此,在较短的DNA区域内(在遗传连锁图上表现1个位点)便有多个SSR标记可供使用,将为定位家蚕重要经济性状基因、分子辅助育种及功能基因研究等提供更多有价值的信息。
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| 关 键 词： | 家蚕 微卫星标记 Scaffold Dll基因 连锁分析 |
| 文章编号： | 0257-4799（2007）02-0187-08 |
| 修稿时间： | 2006-11-30 |
Linkage Analysis of New SSR Markers in Scaffold and Dll Gene in Silkworm |
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| GUO Qiu-Hong,ZHAN Shuai,XIANG Hui,ZHAO Yun-Po,LI Wei-Hua,HUANG Yong-Ping.Linkage Analysis of New SSR Markers in Scaffold and Dll Gene in Silkworm[J].Acta Sericologica Sinica,2007,33(2):187-194. |
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| Authors: | GUO Qiu-Hong ZHAN Shuai XIANG Hui ZHAO Yun-Po LI Wei-Hua HUANG Yong-Ping |
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| Institution: | 1 Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; 2 College of Biotechnology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China |
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| Abstract: | The common polymorphic SSR markers are very useful when the integration of linkage maps constructed from two independent parental combinations should be made.However,it is quite difficult to find those SSR markers which are polymorphic in different parental combinations.In order to solve this problem,we search the correspondence scaffold with the existent SSR sequence by blasting the SSR sequence in the genome of Bombyx mori.Using SSR Hunter 1.3,the neighbor SSR loci were discovered in addition to the original ones.The repeat numbers of those neighbors SSR loci ranged from 6 to 23 were selected to analyzed.Primers were designed in these new SSR region and their polymorphism was detected between the parents of a(7019) female and F1(F50B×7019)male of the BC1 generation.The results showed that there are 6 out of 7 primers derived from chromosome 2 showing polymorphism in the BC1 generation and 2 of them were drawn in the linkage map.Their loci were consistent with the original ones.The genetic distance of two neighbors SSR loci(NS02071 and NS02072) of S0207 is 6.9 cM.Employing a BC1 generation derived from a C108 female and F1(Dazao×C108) male,the two new SSR markers in the same scaffold with Dll gene got the consistent result with a genetic distance of 0.0 cM,which indicated they were overlapping in the linkage map.The diverse markers in one scaffold or contig will be helpful to locate valuable economic character's gene and molecular assistant breeding and position cloning of the other functional genes. |
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| Keywords: | Bombyx mori Microsatellite marker Scaffold Dll gene Linkage analysis |
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