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鸡新城疫血凝和血凝抑制试验实训实习效果分析
引用本文:覃开权.鸡新城疫血凝和血凝抑制试验实训实习效果分析[J].安徽农业科学,2014(18):5881-5882.
作者姓名:覃开权
作者单位:永州职业技术学院,湖南永州,425000
摘    要:目的]对某养鸡场的鸡新城疫抗体进行血清学检测,以有效评估鸡群的免疫状态,制定合理免疫程序。方法]对永州市某养鸡场病鸡进行鸡新城疫血凝(HA)和血凝抑制(HI)试验,检测鸡新城疫抗体水平。结果]在检测的60份血样中鸡新城疫抗体效价为1:8—1:4。鸡新城疫抗体效价≥1:16(410g2)的占13.3%,鸡新城疫抗体效价≥1:8(3lo2)的占21.7%,鸡新城疫抗体效价〈1:4(2log2)的占65%。结论]该鸡场鸡群鸡瘟疫苗免疫失败。

关 键 词:鸡新城疫标准抗原  血凝试验  血凝抑制试验

Training and Practice Effect Analysis of Hemagglutination Test and Hemagglutination Inhibition Test of Chicken Newcastle Disease
QIN Kai-quan.Training and Practice Effect Analysis of Hemagglutination Test and Hemagglutination Inhibition Test of Chicken Newcastle Disease[J].Journal of Anhui Agricultural Sciences,2014(18):5881-5882.
Authors:QIN Kai-quan
Institution:QIN Kai-quan (Yongzhou Vocational Technical College, Yongzhou, Hunan 425000)
Abstract: Objective ] The research aimed to make the serological test on the chicken newcastle disease antibody in a chicken farm, evaluate the immune state of chicken effectively and establish reasonable immune procedure. Method] The hemagglutination test and hemagglutination inhibition test were macle on the diseased chicken in a chicken farm in Yongzhou City to detect the antibody level of chicken newcastle disease. Result] The antibody titer of newcastle disease in 60 detected blood samples was 1:8 - 1: 4. The blood samples with the chicken newcastle disease antibody titer of ≥ 1:16 (4log2 )took 13.3 %, and that with the chicken newcastle disease antibody titer of ≥ 1:8 (3log2 )took 21.7 %, and that with the chicken newcastle disease antibody titer of 1 : 4 (2log2 ) took 65 %. Conclusion ] The immunization of newcastle disease vaccine in this chicken farm failed.
Keywords:Standrd antigen of chicken newcastle disease  Hemagglutination test  Hemagglutination inhibition test
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