Powdery Mildew of Prairie Gentian: Characteristics,Molecular Phylogeny and Pathogenicity |
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| Authors: | OKAMOTO Jun LIMKAISANG Saranya NOJIMA Hidenobu TAKAMATSU Susumu |
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| Institution: | (1) Oita Prefectural Floricultural Research Center, 710–1, Tsurumi, Beppu 874–0844, Japan, JP;(2) Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand, TH;(3) Kagoshima Prefectural Agricultural Experiment Station, 5500, Kamifukumoto, Kagoshima 891–0116, Japan, JP;(4) Faculty of Bioresources, Mie University, 1515, Kamihama, Tsu 514–8507, Japan, JP |
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| Abstract: | In March 1999, we found prairie gentian (Eustoma grandiflorum) infected with powdery mildew in a greenhouse in Oita Prefecture, Japan. Morphological observation revealed that the causal
fungus belongs to the mitosporic genus Oidium subgenus Pseudoidium teleomorph: Erysiphe sensu Braun and Takamatsu (2000)]. Precise taxonomic position of the fungus, however, is uncertain due to lack of the perfect stage.
We determined the nucleotide sequence of the rDNA ITS region of the fungus. Comparison of the sequence with those obtained
from DNA databases of this fungal group revealed that the sequence is identical to those of powdery mildews from garden four-o'clock
(Mirabilis jalapa) and broad bean (Vicia faba). Inoculation of an isolate from garden four-o'clock caused mildew on prairie gentian and broad bean, suggesting that the
prairie gentian mildew originates from garden four-o'clock or broad bean. Molecular phylogenetic analysis indicated a close
relationship of this fungus to Erysiphe baeumleri on Vicia spp. and E. trifolii on Trifolium pratense. From these results, we propose that prairie gentian mildew diverged from a Fabaceae-parasitic ancestor.
Received 14 March 2002/ Accepted in revised form 28 May 2002 |
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| Keywords: | : broad bean Erysiphaceae Eustoma grandiflorum garden four-o'clock internal transcribed spacer Oidium subgenus Pseudoidium |
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