| 头孢噻呋混悬剂体外抗菌活性及抗生素后效应研究 |
| |
| 引用本文： | 王慧,刘伟,陈小军,刘自逵,肖红波,孙志良.头孢噻呋混悬剂体外抗菌活性及抗生素后效应研究[J].中国农业科学,2010,43(7):1493-1499. |
| |
| 作者姓名： | 王慧 刘伟 陈小军 刘自逵 肖红波 孙志良 |
| |
| 作者单位： | （湖南农业大学动物医学院）; |
| |
| 基金项目： | 农业部科技成果转化项目，湖南省科技厅重点项目 |
| |
| 摘 要： | 【目的】评价头孢噻呋混悬剂对兽医临床常见的3种标准菌株的体外抗菌活性及抗生素后效应。【方法】以试管二倍稀释法对3种标准菌株进行最低抑菌浓度(MIC)和最小杀菌浓度(MBC)的测定;采用菌落计数法绘制3种标准菌株的时间-杀菌曲线(KCs);采用平板计数法测定了头孢噻呋混悬剂对3种标准菌株的抗生素后效应(post-antibiotic effect,PAE)。【结果】头孢噻呋混悬剂对大肠杆菌、放线杆菌及猪兽疫链球菌的MIC值分别为:0.076、0.304、0.00475μg·mL-1;MBC值分别为:0.61、0.304、0.00475μg·mL-1;头孢噻呋混悬剂对放线杆菌的杀菌速率最快,对链球菌次之;头孢噻呋混悬剂对大肠杆菌PAE较短,呈现非浓度依赖性,对放线杆菌的PAE较长,呈明显的浓度依赖性,而对猪兽疫链球菌呈现部分浓度依赖性。【结论】头孢噻呋混悬剂具有强效、速效、持效的杀菌作用,可广泛应用于兽医临床。
|
| 关 键 词： | 头孢噻呋混悬剂 最低抑菌浓度 最低杀菌浓度 时间-杀菌曲线 PAE |
| 收稿时间： | 2009-02-22; |
The in vitro Antibacterial Activities and Post-Antibiotic Effects of the Ceftiofur Suspension |
| |
| WANG Hui,LIU Wei,CHEN Xiao-jun,LIU Zhi-kui,SUN Zhi-liang.The in vitro Antibacterial Activities and Post-Antibiotic Effects of the Ceftiofur Suspension[J].Scientia Agricultura Sinica,2010,43(7):1493-1499. |
| |
| Authors: | WANG Hui LIU Wei CHEN Xiao-jun LIU Zhi-kui SUN Zhi-liang |
| |
| Institution: | WANG Hui1,LIU Wei1,CHEN Xiao-jun1,LIU Zhi-kui2,SUN Zhi-liang1 (1College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,2Engineering Research Center of Veterinary Drugs of Hunan Province,Changsha 410311) |
| |
| Abstract: | Objective] The objective of the study was to evaluate the in vitro antibacterial activity and post-antibiotic effect of ceftiofur suspension on three standard bacteria strains in veterinary clinic. Method] Minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were tested by double dilution method to three standard bacteria strains. Time-kill curve was drew according to colony count. Post-antibiotic effect (PAE) was determined by plate count. Result] MIC value of ceftiofur suspension to E. Coli, Actinobacillus and Streptococcus zooepidemicus was 0.076, 0.304, and 0.00475 μg·mL~(-1) respectively. MBC value was 0.61, 0.304, and 0.00475 μg·mL~(-1) respectively. Sterilization rate of ceftiofur suspension was the fastest to Actinobacillus, followed by Streptococcus. PAE of ceftiofur suspension was relatively short to E.coli, which illustrated that it was nonconcentration dependent. PAE was obvious longer to Actinobacillus, which showed that it was concentration dependent, however, it revealed part concentration dependent to streptococcus. Conclusion] Ceftiofur suspension can be widely used in veterinariy clinic due to its strong, immediate, and persistent sterilization. |
| |
| Keywords: | PAE ceftiofur suspension minimal inhibitory concentration minimum bactericidal concentration time-kill curve PAE |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
| 点击此处可从《中国农业科学》下载免费的PDF全文 |
|
