| 高氟低碘对老年大鼠肝脏细胞DNA损伤的影响 |
| |
| 引用本文： | 宁红梅,葛亚明,安志兴,银梅,刘俊伟,陈永耀,崔艳红,钟华.高氟低碘对老年大鼠肝脏细胞DNA损伤的影响[J].安徽农业科学,2010,38(4):2115-2117. |
| |
| 作者姓名： | 宁红梅 葛亚明 安志兴 银梅 刘俊伟 陈永耀 崔艳红 钟华 |
| |
| 作者单位： | 河南科技学院,河南新乡,453003 |
| |
| 基金项目： | 博士启动基金(06007);;校骨干教师基金(200711) |
| |
| 摘 要： | 目的]研究高氟低碘对大鼠肝脏细胞DNA损伤的影响。方法]离乳Wistar大鼠21只,随机分为4组：①CK（5只）,饲喂大鼠标准饲料,饮自来水;②高氟组（5只）,饲喂大鼠正常饲料,饮100mg/L氟化钠（NaF）去离子水;③低碘组（5只）,饲喂低碘饲料（定做）,饮去离子水;④高氟低碘组（6只）,饲喂低碘饲料,饮100mg/L氟化钠（NaF）去离子水。在大鼠20月龄时,处死取肝脏组织,检查其DNA的损伤情况。结果]与①CK相比,②、③和④的老年大鼠肝细胞拖尾率显著增高（P〈0.01）;②、③和④的老年大鼠肝细胞的拖尾长度亦显著增长（P〈0.01）;肝细胞DNA损伤分级结果显示,②（高氟组）老年大鼠肝细胞DNA损伤主要是Ⅱ级、Ⅲ级。③（低碘组）肝细胞DNA几乎全部损伤,主要是Ⅲ级损伤。④（高氟低碘组）的细胞损伤以Ⅲ级损伤为主。结论]高氟、低碘、高氟低碘都影响了大鼠肝脏细胞DNA的损伤。
|
| 关 键 词： | 高氟低碘 老年大鼠 肝脏 DNA损伤 单细胞凝胶电泳 |
Effects of High Fluoride and Low Iodine on DNA Damage in Liver Cells in Aged Rats |
| |
| NING Hong-mei et al.Effects of High Fluoride and Low Iodine on DNA Damage in Liver Cells in Aged Rats[J].Journal of Anhui Agricultural Sciences,2010,38(4):2115-2117. |
| |
| Authors: | NING Hong-mei |
| |
| Institution: | College of Animal Science;Henan Institute of Science and Technology;Xinxiang;Henan 453003 |
| |
| Abstract: | Objective] The research aimed to study the effects of high-fluoirde and low-iodine on DNA damage of liver cells in rats. Method] 21 weaned Wistar rats were randomly divided into 4 groups, including ① CK (feeding 5 rats with standard diet and tap water), ② high-F group (feeding 5 rats with normal diet and 100 mg/L NaF deionized water), ③ low-I group (feeding 5 rats with low-I diet and deionized water) and ④ high-F and low-I group (feeding 6 rats with low-I diet and 100 mg/L NaF deionized water). On the 20th month, rats were killed and their liver tissues were taken to detect the situations of DNA damage. Result] Compared with CK, the tailing rate of liver cells in aged rats in groups ②, ③ and ④ was significantly increased (P＜0.01) and the tail length of liver cells in aged rats in groups ②, ③ and ④ was longer(P＜0.01). The classification results of DNA damage in liver cells indicated that DNA damage in liver cells in aged rats in group ② were mainly Ⅱ-class and Ⅲ -class. DNA in liver cells in group ③ were almost damaged, being mainly Ⅲ-class. The cell damage in group ④ was Ⅲ -class. Conclusion] High-F, low-I, high-F and low-I all affected DNA damage in liver cells in rats. |
| |
| Keywords: | High-F and low-I Aged rats Liver DNA damage Single cell gel electrophoresis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
