| 碱性蛋白酶酶解丰年虫卵蛋白工艺条件优化 |
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| 引用本文： | 彭鑫华,张建新,聂 路,王 欣.碱性蛋白酶酶解丰年虫卵蛋白工艺条件优化[J].西北农业学报,2011,20(12):197-202. |
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| 作者姓名： | 彭鑫华 张建新 聂 路 王 欣 |
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| 作者单位： | 西北农林科技大学食品科学与工程学院; |
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| 基金项目： | 国家级大学生创新资助项目(2009-12) |
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| 摘 要： | 以丰年虫卵为原料,利用碱性蛋白酶酶解制备营养价值更高的多肽,并对其水解参数进行优化.以水解度(DH)为指标,通过单因素试验研究酶解时间、酶解温度、pH、底物质量分数及加酶量等因素对碱性蛋白酶酶解丰年虫卵蛋白质过程的影响.在此基础上利用3因素(pH、酶解温度和加酶量)中心组合试验设计,得出最优酶解时间为150 min,底...
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| 关 键 词： | 丰年虫卵 碱性蛋白酶 中心组合设计 水解度 |
Optimization of Enzymatic Hydrolysis for Artemla salina Egg Proteins by Alkaline Protease |
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| PENG Xinhu,ZHANG Jianxin,NIE Lu and WANG Xin.Optimization of Enzymatic Hydrolysis for Artemla salina Egg Proteins by Alkaline Protease[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(12):197-202. |
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| Authors: | PENG Xinhu ZHANG Jianxin NIE Lu and WANG Xin |
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| Institution: | PENG Xinhua,ZHANG Jianxin,NIE Lu and WANG Xin(College of Food Science and Engineering,Northwest A&F University,Yangling Shaanxi 712100,China) |
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| Abstract: | In order to make the peptides nutrient value much better,Artemia salina egg protein was hydrolyzed by alkaline protease.Conditions of enzymatic reaction were determined and optimized.Using degree of hydrolysis(DH) as an indicator,single factor experiments were conducted to optimize the effect of enyzmatic hydrolysis parameters,including reaction time,temperature,pH,substrate concentration and dosage of protease,on DH of Artemia salina egg by alkaline protease.Then,a central composite design(CCD) with 3 fact... |
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| Keywords: | Artemia salina eggs Enzymatic hydrolysis Alkaline protease Central composite design Degree of hydrolysis |
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