High-throughput <Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated barley transformation |
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| Authors: | Joanne G Bartlett Sílvia C Alves Mark Smedley John W Snape Wendy A Harwood |
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| Institution: | (1) Department of Crop Genetics, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK;(2) Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK |
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| Abstract: | Background Plant transformation is an invaluable tool for basic plant research, as well as a useful technique for the direct improvement
of commercial crops. Barley (Hordeum vulgare) is the fourth most abundant cereal crop in the world. It also provides a useful model for the study of wheat, which has
a larger and more complex genome. Most existing barley transformation methodologies are either complex or have low (<10%)
transformation efficiencies. |
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