| 地锦草总黄酮提取工艺研究 |
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| 引用本文： | 张建超,陈小军,徐波,刘伟,王慧,孙志良.地锦草总黄酮提取工艺研究[J].中兽医医药杂志,2009,28(4):17-21. |
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| 作者姓名： | 张建超 陈小军 徐波 刘伟 王慧 孙志良 |
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| 作者单位： | 1. 湖南农业大学动物医学院基础兽医,湖南长沙,410128
2. 湖南农业大学动物医学院基础兽医,湖南长沙,410128;湖南省兽药工程技术研究中心 |
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| 基金项目： | 湖南省科学技术厅项目 |
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| 摘 要： | 运用正交试验优化超声粉碎地锦草细胞的最佳条件,采用超声-加热回流-大孔树脂吸附的工艺流程提取分离纯化地锦草总黄酮。结果表明,超声波提取地锦草总黄酮的最佳工艺以料液比为1∶20,70%的乙醇为提取溶剂,60℃超声提取35min,影响超声提取效果大小的因素依次为提取时间〉溶剂浓度〉料液比〉提取温度;100g地锦草干燥全草提取纯化得到黄褐色粉末3.679g,经多种化学试剂鉴定,均呈明显的黄酮反应。
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| 关 键 词： | 地锦草 超声波提取 总黄酮 |
Study on extraction process of total flavonoids in Euphorbiae humifusa Willd. |
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| ZHANG Jian-chao,CHEN Xiao-jun,XU Bo,LIU Wei,WANG Hui,SUN Zhi-liang.Study on extraction process of total flavonoids in Euphorbiae humifusa Willd.[J].Journal of Traditional Chinese Veterinary Medicine,2009,28(4):17-21. |
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| Authors: | ZHANG Jian-chao CHEN Xiao-jun XU Bo LIU Wei WANG Hui SUN Zhi-liang |
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| Institution: | ZHANG Jian-chao,CHEN Xiao-jun,XU Bo,LIU Wei,WANG Hui,SUN Zhi-ling(College of Veterinary Medicine,Hunan Agricultural University,Changsha Hunan,410128,China) |
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| Abstract: | To optimize the ultrasonic conditions with orthogonal test, then to extract, separate and purify the total flavonoids with the method of ultrasonication-heating reflux-macroreticular resin adsorbing. The results showed that the optimal ultrasonic conditions was solid-liquid extracting time as 35 min. The factors which impact on the efficiency of ultrasonic extraction were as follows: extracting time＞solvent concentration＞solid-liquid ratio＞temperature. 3.679 g brown powder was purified from 100 g whole dry Euphorbiae humifusa Willd., identified by a variety of chemical reagents, which showed clearly flavone reaction. |
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| Keywords: | Euphorbiae humifusa Willd ultrasonic total flavonoids |
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