| 31个种源东部白松SRAP指纹图谱分析 |
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| 引用本文： | 王骞春,冯健,陈罡,陆爱君,于世河,杨鹤.31个种源东部白松SRAP指纹图谱分析[J].辽宁林业科技,2011(6):5-7,60. |
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| 作者姓名： | 王骞春 冯健 陈罡 陆爱君 于世河 杨鹤 |
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| 作者单位： | 辽宁省林业科学研究院,辽宁沈阳,110032 |
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| 基金项目： | 辽宁省科学技术计划重大项目“林木遗传资源保护及良种选育”(2011207002) |
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| 摘 要： | 采用8对SRAP引物对加拿大31个种源的东部白松进行分析,获得104个扩增位点,多态性位点79个,平均多态性水平76%,每对引物产生等位基因7～22个,条带分布100～1 000 bp。各种源相互间相似系数为0.653 8～0.951 9,平均0.830 5。引物组合ME1-EM6与ME7-EM7可将31个种源区分开。
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| 关 键 词： | 东部白松 种源 指纹图谱 SRAP |
SRAP fingerprinting analysis of 31 provenances of Pinus strobus |
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| WANG Qian-chun,FENG Jian,CHEN Gang,LU Ai-jun,Yu Shi-he,YANG He.SRAP fingerprinting analysis of 31 provenances of Pinus strobus[J].Liaoning Forestry Science and Technology,2011(6):5-7,60. |
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| Authors: | WANG Qian-chun FENG Jian CHEN Gang LU Ai-jun Yu Shi-he YANG He |
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| Institution: | WANG Qian-chun,FENG Jian,CHEN Gang,LU Ai-jun,Yu Shi-he,YANG He(Liaoning Academy of Forestry Science,Shenyang 110032,China) |
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| Abstract: | Based on the analysis of 31 provenances of Pinus strobus from Canada by using 8 pairs of SRAP markers,104 loci were obtained totally,and 79 were polymorphic loci,the average polymorphism level was 76%,7～22 alleles were produced by each pair of primers,and the fragment size of PCR products ranged from 100 bp to 1 000 bp.The similar coefficient among all the provenances was between 0.653 8 and 0.951 9 with an average of 0.830 5.According to the result of data analysis,primer combinations ME1-EM6 and EM7-ME7 could be used to identify the 31 Pinus strobus. |
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| Keywords: | Pinus strobus provenances fingerprinting SRAP |
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