Mineralization of bacteria and fungi in chloroform-fumigated soils |
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| Authors: | JPE Anderson KH Domsch |
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| Institution: | Institut für Bodenbiologie, FAL, Bundesallee 50, D 3300 Braunschweig, Fed. Rep. Germany |
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| Abstract: | It has been suggested by others that the size of the flush of mineralization caused by CHC13 fumigation can be used to estimate the amount of microbial biomass in soils. Calculation of biomass from the flush requires that the proportion of CHCl3-killed cell C mineralized be known. To determine this proportion, 15 species of 14C]labelled fungi and 12 species of 14C]labelled bacteria were added to four types of soil and these were fumigated for 24 h with CHC13, reinoculated with unfumigated soil, and incubated at 22°C for 10 days. The average percentage mineralization of the fungi was 43.7 ± 5.3, while the average for the bacteria was 33.3 ± 9.9. Using a 1:3 ratio for distribution of total biomass between the bacterial and fungal populations, respectively, it was calculated that the average mineralization of both types of cells was 41.1%. In experiments conducted to determine if CHC13 vapour alters stabilized microbial metabolites or dead microbial cells in a manner which makes them more susceptible to degradation, it was found that both fumigated and unfumigated dead fungal materials mineralized to the same extent in soil during 10 days of incubation. |
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