Detection of soluble Rhizobium japonicum antigens in soil by immunodiffusion |
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| Authors: | Robert J Kremer George H Wagner |
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| Institution: | Department of Agronomy, University of Missouri, Columbia, Missouri 65201 U.S.A. |
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| Abstract: | Innovative techniques for quantifying rhizobia in soil are needed. Immunodiffusion was adapted for use in evaluating four strains of Rhizobium japonicum incubated in soil. Reproducible precipitin bands were obtained using uniform soil cores 5 mm dia × 4 mm length collected with a cork borer. A flocculent of Ca(OH)2 and MgCO3 added to the microcores enhanced band formation. Clear distinct bands developed from populations in soil of 1 × 108 cells g?1 or greater. The complete set of bands which characterized cells cultured on artificial media did not develop from cells inoculated into soil, but at least one detectable band was always observed. Parameters of intensity value and band positioning were related to the population in the soil. Detection by immunodiffusion of a series of rhizobial populations in soil decreased with time almost linearly as the initial population decreased. Direct diffusion of antigens from soil and the development of specific precipitin bands provides a new approach to studying soil microorganisms. |
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