| 喀西茄抗病基因同源序列的分离与分析 |
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| 引用本文： | 庄勇,王述彬.喀西茄抗病基因同源序列的分离与分析[J].分子植物育种,2009,7(6):1223-1228. |
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| 作者姓名： | 庄勇 王述彬 |
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| 作者单位： | 江苏省农业科学院蔬菜作物研究所,南京,210014 |
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| 基金项目： | 江苏省自然科学基金，江苏省科技基础设施建设计划，江苏省农业科技自主创新资金[CX(09)119]共同资助 |
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| 摘 要： | 本研究根据已知植物抗病基因的NBS-LRR保守结构域设计了1对简并引物,对野生茄子喀西茄(Solanum khasianum)中抗病基因的同源序列(resistance gene analogs,RGAs)进行克隆和分析.结果表明,所获得的11个抗病基因同源序列都是Non-TIR-NBS-LRR类抗病基因,聚类分析将其分为5个亚类.由其核酸序列推导的氨基酸序列与已知抗病基因(N、L6、M、Prf、Gpa2和RPM1)相应区域的相似性为21.7%～52.4%.BlastX分析发现,SkRGA4和SkRGA10与辣椒抗根结线虫基因有着很高的同源性,SkRGA3和SkRGA6与野生马铃薯的抗晚疫病基因有着很高的同源性.
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| 关 键 词： | 喀西茄(Solanumkhasianum) 抗病基因 NBS-LRR域 |
Isolation and Analysis of Resistance Gene Analogs from Solanum khasianum |
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| Zhuang Yong,Wang Shubin.Isolation and Analysis of Resistance Gene Analogs from Solanum khasianum[J].Molecular Plant Breeding,2009,7(6):1223-1228. |
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| Authors: | Zhuang Yong Wang Shubin |
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| Institution: | Zhuang Yong Wang Shubin Institute ofVegetable Crops,Jiangsu AcademyofAgricultural Sciences,Nanjing,210014 |
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| Abstract: | In this paper,we cloned and analyzed the resistance gene analogs (RGAs) from himalaya nightshade (Solanum khasianum),a kind of wild eggplant,by employing a pair of degenerate primers designed based on nu-cleotide binding site (NBS)-leucine rich repeat (LRR) conserved domain of reported resistant genes from other plants.The results showed that the eleven RGAs obtained might assigned to Non-TIR-NBS-LRR type of resistant genes.Clustering analysis demonstrated that the eleven RGAs can be classified into five di... |
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| Keywords: | Himalaya nightshade (Solarium khasianum) Resistance gene NBS-LRR domain |
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