| 应用SSR标记技术鉴定登海11号玉米杂交种纯度的研究 |
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| 引用本文： | 李群,李汝玉,颜廷进,谭振新.应用SSR标记技术鉴定登海11号玉米杂交种纯度的研究[J].种子,2004,23(10):21-23. |
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| 作者姓名： | 李群 李汝玉 颜廷进 谭振新 |
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| 作者单位： | 农业部农作物种质监督检验测试中心,山东,济南,250100 |
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| 摘 要： | 以登海11号玉米杂交种及其亲本和9对玉米SSR位点引物为材料,筛选出了适合该杂交种纯度鉴定的SSR位点引物;并对利用幼苗和干种子为材料提取DNA的效果进行了比较,建立了一套利用SSR标记技术进行玉米杂交种纯度鉴定的技术规程.该操作程序简单、快速、对仪器设备要求较低,易于推广.
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| 关 键 词： | 玉米杂交种 DNA提取 纯度 SSR标记技术 |
Application of SSR Marker Technique to Purity Testing of Denghai No.11 Maize |
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| Li Qun,Li Ruyu,Yan Tingjin,Tan Zhenxin.Application of SSR Marker Technique to Purity Testing of Denghai No.11 Maize[J].Seed,2004,23(10):21-23. |
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| Authors: | Li Qun Li Ruyu Yan Tingjin Tan Zhenxin |
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| Abstract: | In this study, 9 SSR primer pairs were screened to search for the SSR loci that are suitable for testing the purity of Denhai No.11 maize hybrid, which lack a reliable laboratory method for purity testing. A polymorphic SSR loci, phil 053, has been found that could be applied to test the purity of this hybrid. A procedure featuring inexpensive equipment and ease of use has been set up for testing maize hybrid purity using SSR marker. |
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| Keywords: | Maize hybrid DNA extraction Purity SSR marker |
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