Development of 11 microsatellite markers in <Emphasis Type="Italic">Pinus parviflora</Emphasis> by the dual-suppression technique and next-generation sequencing |
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| Authors: | Yukiko Sakata Bekku Hiroyuki Kurokochi Yu Matsuki Engkong Tan Shuichi Asakawa Satoshi Imura Chunlan Lian |
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| Institution: | 1.Department of Primary Education,Tsuru University,Yamanashi,Japan;2.Asian Natural Environmental Science Center,The University of Tokyo,Tokyo,Japan;3.Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences,The University of Tokyo,Tokyo,Japan;4.National Institute of Polar Research,Tokyo,Japan;5.Field Science Center, Graduate School of Agricultural Science Forest Ecology,Tohoku University,Osaki,Japan |
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| Abstract: | Pinus parviflora Sieb. et Zucc. var. parviflora is a coniferous tree species distributed in mountainous areas of temperate zones from southern Tohoku to the Kyushu area in Japan. Some P. parviflora populations have become small and endangered due to massive death resulting from pine wilt disease, scab canker, and presumably climate change. We developed 11 microsatellite markers for P. parviflora by the dual-suppression technique and next-generation sequencing using 32 individuals of P. parviflora collected from Aokigahara at the foot of Mt. Fuji. The number of alleles for each locus ranged from two to 10. The averages of observed and expected heterozygosities were 0.61 and 0.59, respectively. These markers will become powerful tools for assessing genetic diversity, genetic connectivity, and genetic structure in P. parviflora populations, which will facilitate our understanding and conservation of P. parviflora. |
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