Characterization of tolerance to Fusarium oxysporum f.sp., cubense infection in banana using suppression subtractive hybridization and gene expression analysis |
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| Institution: | 1. Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore 560089, India;2. Division of Fruit Crops, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore 560089, India;1. University of Idaho, Department of Plant, Soil and Entomological Sciences, Moscow, ID 83844-2339, USA;2. Oregon State University, Department of Crop and Soil Science, Corvallis, OR 97331, USA;1. Environmental Toxicology Research Center, Korea Institute of Toxicology (KIT), Jinju 52834, Republic of Korea;2. Division of Applied Life Science (BK21Plus) and Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea;3. Center for Research Facilities, Gyeongsang National University, Jinju 52828, Republic of Korea;4. United States Department of Agriculture-Agriculture Research Service, Wheat Health, Genetics and Quality Research Unit, Pullman, WA 99164, USA;1. Laboratory of Plant Pathology and Biotechnology, Kochi University, Nankoku, Kochi, 783-8502, Japan;2. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan;3. Research Institute of Molecular Genetics, Kochi University, Nankoku, Kochi, 783-8502, Japan;1. N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47, Moscow, 119991, Russian Federation;2. School of Biology, M. V. Lomonosov Moscow State University, Moscow, 119991, Russian Federation;3. All-Russian Collection of Microorganisms (VKM), G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russian Federation;1. National Glycoengineering Research Center, Shandong University, No. 72 Binhai Road, Qingdao, 266237, China;2. State Key Lab of Microbial Technology, Shandong University, No. 72 Binhai Road, Qingdao, 266237, China |
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| Abstract: | Identification of defense response genes in the host is one of the most essential steps in understanding disease resistance mechanisms in plants. In this study, suppression subtractive hybridization (SSH) library was constructed to study the genes involved in response to fusarium wilt disease in banana. Here cDNAs from a tolerant genotype Musa acuminata spp. burmannicoides ‘Calcutta-4’ infected by Fusarium oxysporum f.sp., cubense were used as tester and cDNAs from uninfected ‘Calcutta-4’ as driver population. After hybridization and cloning, EST library of 83 non-redundant clones were obtained. Based on sequence analysis and homology search in NCBI database the clones were assigned to different functional categories. The expression pattern of selected eight defense related genes namely peroxidase, glutaredoxin, polyphenol oxidase, glutamate synthase, S-adenosyl methionine synthetase, 14-3-3, heat shock protein, mannose binding lectin were analyzed through real-time PCR in contrasting genotypes. It was observed that the expression of these genes during initial progression of disease was found to be higher in tolerant genotype ‘Calcutta-4’ than in susceptible genotype ‘Kadali’. |
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