High montmorillonite content may affect soil microbial proteomic analysis |
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| Institution: | 1. Regional Centre of Advanced Technologies and Materials, Palacky University, Slechtitelu 27, 783 71 Olomouc, Czech Republic;2. Department of Physical Chemistry, Faculty of Science, Palacky University, 17. listopadu 1192/12, 771 46 Olomouc, Czech Republic;3. Department of Nanobiotechnology, Biology Centre, ISB, CAS, Na Sadkach 7, 370 05 Ceske Budejovice, Czech Republic;1. Dpto. Edafoloxía y Química Agrícola, Facultade de Bioloxía, Universidade de Santiago de Compostela, Rúa Lope Gómez de Marzoa s/n, Campus Vida, 15782 Santiago de Compostela, Spain;2. Instituto de Recursos Naturales y Agrobiología de Sevilla, Consejo Superior de Investigaciones Científicas, IRNAS-CSIC., Avda. Reina Mercedes 10, 41012 Sevilla, Spain;3. Institute of Bio- and Geosciences, Agrosphere (IBG-3), Forschungszentrum Jülich GmbH., Wilhelm-Johnen-Straße, 52428 Jülich, Germany |
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| Abstract: | We studied the effects of high montmorillonite content in soil on the proteomic analysis of Cupriavidus metallidurans CH34 inoculated into model soils, containing a montmorilonite gradient. Bacterial proteomic analysis was conducted by two-dimensional gel electrophoresis (2-DE) coupled to mass spectrometry. The results showed that increasing the montmorillonite content in artificial soil the bacterial viability did not affect but the amount of extracted proteins and the number of protein spots in 2-DE decreased. Higher soil montmorillonite content also affected the protein identification, likely due to montomrillonite-induced conformational changes in proteins or degradation. Therefore the development of soil proteomics needs to increase the studies of interaction between protein and soil components as clays or humic substances. This experiment showed how the use of a model study can be an help to achieve more information about the complexity of soil and the fate of proteins in soil. |
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| Keywords: | Soil proteomics Protein–clay interactions Protein extraction from soil Protein identification |
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