Non-destructive sampling procedure for biochemical or gene expression studies on post-harvest physiological deterioration of cassava roots |
| |
| Institution: | 1. Consorcio Latinoamericano para Investigación y Desarrollo de la Yuca (CLAYUCA), Apartado Aéreo 6713, Cali, Colombia;2. International Center for Tropical Agriculture (CIAT), Apartado Aéreo 6713, Cali, Colombia;1. Cyprus University of Technology, Department of Agricultural Sciences, Biotechnology & Food Science, 3603, Lemesos, Cyprus;2. Institute of Plant Breeding and Genetic Resources, Department of Deciduous Fruit Trees, Hellenic Agricultural Organization ''Demeter'', 38 R.R. Station, 59035, Naoussa, Greece;3. Aristotle University of Thessaloniki, School of Agriculture, 54124, Thessaloniki, Greece;4. Alexander Technological Educational Institute, Department of Agricultural Technology, 57400, Sindos-Thessaloniki, Greece;5. Technological Educational Institute of Crete, Department of Agricultural Technology, 71004, Estavromenos, Heraklion, Crete, Greece;1. Department of Neurosurgery, Austin Health, 145 Studley Road, Melbourne 3084, Australia;2. Department of Radiology, Austin Health, 145 Studley Road, Melbourne 3084, Australia;3. Department of Anatomical Pathology, Austin Health, 145 Studley Road, Melbourne 3084, Australia;1. Grazing Ecology Research Group, Federal University of Rio Grande do Sul, Porto Alegre, Brazil;2. Brazilian Agricultural Research Corporation, Bagé, Brazil;3. Federal University of Pampa, Itaqui, Brazil;4. Department of Agricultural Diagnosis and Research, São Gabriel, Brazil;5. Department of Animal Nutrition and Management, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Uppsala, Sweden;1. IFREMER, Centre de Bretagne, REM/EEP, Laboratoire Environnement Profond, F-29280 Plouzané, France;2. IFREMER, Centre de Bretagne, REM/EEP, Laboratoire de Microbiologie des Environnement Extrêmes, F-29280 Plouzané, France;3. Université de Bretagne Occidentale, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France;4. CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France;5. IFREMER, Centre de Bretagne, REM/EEP, Laboratoire Cycles Géochimiques et ressources, F-29280 Plouzané, France;6. School of Earth and Atmospheric Sciences, Georgia Institute of Technology, Atlanta, GA 30332, United States;7. Laboratoire des Sciences du Climat et de l’Environnement, UMR 8212 CEA-CNRS-UVSQ and IPSL, Av. de la Terrasse, 91190 Gif sur Yvette, France;2. Western Cape Department of Agriculture, Outeniqua Research Farm, George 6530, South Africa |
| |
| Abstract: | Cassava (Manihot esculenta Crantz) roots spoil 2–3 days after harvest due to post-harvest physiological deterioration (PPD), which had remained an unsolved problem until recent reports of genetic variation for tolerance to it. PPD is a genetically active, oxidative process triggered when the harvested roots are separated from their mother plant. The short shelf life of harvested roots results in large losses and high transport and marketing costs. Recent reports on positive genetic variation for tolerance to PPD will facilitate breeding for extended shelf life of the roots and a better understanding of the biochemical and genetic events leading to PPD. However, PPD scoring is difficult and prone to large experimental errors. It is often the case that roots from the same plant can have 0 and 100% PPD score due to injuries during the harvest process, variation in dry matter content and, most likely, other variables yet to be identified. Therefore, sampling a root for biochemical or genetic studies and measuring PPD in a different root, is not a reliable approach. A device has been developed and tested for the possibility of extracting a core of root parenchyma, filling the space with melted paraffin (to reduce oxygen availability), and then one or two weeks later visually quantifying PPD in the same root. Sampling the roots did not have any significant effect on PPD suggesting that the protocol can be used for biochemical composition and gene expression studies related to the causes of PPD and the possibilities of developing tolerance to it. |
| |
| Keywords: | Gene expression Genetic tolerance Post-harvest losses Shelf life |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
