Protecting effect of vitamin E supplementation on submaximal exercise-induced oxidative stress in sedentary dogs as assessed by erythrocyte membrane fluidity and paraoxonase-1 activity |
| |
| Authors: | Motta S Letellier C Ropert M Motta C Thiébault J J |
| |
| Institution: | aPhysiology and Therapeutic Department, National Veterinary School of Lyon, 1, Avenue Bourgelat, 69280 Marcy l’Etoile, France;bCHU Hospital SUD, Laboratory of Biochemistry, 35056 Rennes, France |
| |
| Abstract: | The aim of this placebo-controlled study was to investigate the effects of oral vitamin E supplementation for 10 weeks on exercise-induced oxidative damage in untrained dogs. Eight dogs were randomly assigned to a supplementation (n = 4) or control (n = 4) group and underwent two isolated submaximal exercise sessions, 10 weeks apart. Blood was collected during each session to measure erythrocyte membrane fluidity (EMF), paraoxonase-1 (PON1) activity, plasma malondialdehyde (MDA) and vitamin E concentrations. These biomarkers were measured in venous blood samples collected before (t0), just after (t, EMF only) and 1 d (t + 1 d) and 7 d (t + 7 d) after the dogs ran on a treadmill.Prior to vitamin E supplementation, exercise induced a significant decrease in PON1 activity, EMF, vitamin E concentration and a significant increase in MDA concentration at t + 1 d. After a 10 week vitamin E supplementation period, these exercise-induced changes in PON1 activity, EMF and MDA concentration were still significant in the control group, but not in the supplemented group. These results suggested that vitamin E supplementation had a protective effect on submaximal exercise-induced oxidative damage in sedentary dogs. |
| |
| Keywords: | Dogs Exercise Paraoxonase-1 Membrane fluidity Vitamin E |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
