| 鹰嘴豆EST资源中SSR的信息分析 |
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| 引用本文： | 王显生,贾钰莹,顾汉艳,何小玲,张桦,张巨松,石书兵,李建贵,麻浩.鹰嘴豆EST资源中SSR的信息分析[J].湖南农业大学学报(自然科学版),2010,36(2):137-141. |
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| 作者姓名： | 王显生 贾钰莹 顾汉艳 何小玲 张桦 张巨松 石书兵 李建贵 麻浩 |
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| 作者单位： | 1. 南京农业大学,作物遗传与种质创新国家重点实验室,江苏,南京,210095
2. 新疆农业大学,农业生物技术重点实验室,新疆,乌鲁木齐,830052 |
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| 基金项目： | 国家科技支撑计划项目，国家自然科学基金项目，国家博士后科学基金项目，高等学校学科创新引智计划项目，科技支疆项目 |
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| 摘 要： | 从NCBI公共数据库下载获得27 904条鹰嘴豆表达序列标签EST,去除其中低质量的和冗余序列后得到全长为6 198 092 bp的11 224 条无冗余EST.利用SSRIT(Simple sequence repeat identification tool)软件共在这些序列中发掘出982个SSR,它们分布于865条EST中,出现频率为8.69%,平均长度为15.58 bp,平均距离为6.31 kb.在鹰嘴豆EST-SSR中,二核苷酸重复是最主要的重复类型(66.90%),其次是三核苷酸重复类型(30.75%),出现最多的重复基元类型是TA/AT(24.95%).
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| 关 键 词： | 鹰嘴豆 表达序列标签-简单重复序列 频率 特性 |
| 收稿时间： | 2009/9/1 0:00:00 |
| 修稿时间： | 2009/12/14 0:00:00 |
Information analysis of EST resource of chickpea |
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| Wang Xianshneg,JIa Yuying,Gu Hanyan,He Xiaoling,Zhang Hu,Zhang Jusong,Shi Shubing,Li Jiangui and Ma Hao.Information analysis of EST resource of chickpea[J].Journal of Hunan Agricultural University,2010,36(2):137-141. |
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| Authors: | Wang Xianshneg JIa Yuying Gu Hanyan He Xiaoling Zhang Hu Zhang Jusong Shi Shubing Li Jiangui and Ma Hao |
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| Institution: | Nanjing Agricultural University,Nanjing Agricultural University,Nanjing Agricultural University,Nanjing Agricultural University,Xinjiang Agricultural University,Xinjiang Agricultural University,Xinjiang Agricultural University,Xinjiang Agricultural University,Nanjing Agricultural University |
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| Abstract: | 27 904 ESTs of chickpea (Cicer arietinum L.) were downloaded from the database of NCBI and some redundant or low quality sequences were removed. Finally, 11 224 non-redundant ESTs with total length of 6 198 092 bp were obtained. SSRIT (Simple sequence repeat identification tool), a web tool for SSR hunting, was used to analyze the sequences. The results showed that a total of 982 SSRs distributed in 865 ESTs were detected, with the frequency for the non-redundant ESTs being 8.69%. The average length and distribution distance of the EST-SSRs were about 15.58 bp and 6.31 kb. Among the different SSRs ranging from 2 to 7 bp, dinucleotide repeats were most abundant (66.90%), followed by trinucleotide repeats (30.75%). Among 83 identified motif types, TA/AT had the highest EST frequency (24.95%). The results would contribute to the development of EST-SSR markers in chickpea. |
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| Keywords: | chickpea EST-SSR frequency characteristics |
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