Identification of Staphylococcus hyicus by polymerase chain reaction mediated amplification of species specific sequences of superoxide dismutase A encoding gene sodA |
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| Authors: | Voytenko A V Kanbar T Alber J Lämmler C Weiss R Prenger-Berninghoff E Zschöck M Akineden O Hassan A A Dmitrenko O A |
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| Institution: | Kovalenko Research Institute of Experimental Veterinary Medicine, Kurskaya Str. 4, 308002 Belgorod, Russia. |
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| Abstract: | A species specific PCR test, based on manganese-dependent superoxide dismutase A encoding gene sodA, was developed for the identification of Staphylococcus hyicus, an important bacterial pathogen in pigs. The designed primers allowed a rapid and reliable identification of phenotypically characterized S. hyicus, isolated in Russia, Germany and Denmark. No cross reactivities could be observed investigating staphylococcal reference strains representing 18 different species and subspecies. The use of the described primers might improve a future diagnosis of this bacterial pathogen. |
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