| 毒死蜱降解细菌WJl-063的鉴定及酶促降解特性 |
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| 引用本文： | 王利,余贤美,贺春萍,郑服丛.毒死蜱降解细菌WJl-063的鉴定及酶促降解特性[J].热带作物学报,2009,30(3):357-361. |
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| 作者姓名： | 王利 余贤美 贺春萍 郑服丛 |
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| 作者单位： | 1. 海南大学环境与植物保护学院,海南儋州,571737;中国热带农业科学院环境与植物保护研究所,海南儋州,571737
2. 中国热带农业科学院环境与植物保护研究所,海南儋州,571737 |
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| 基金项目： | 中央级公益性科研院所基本科研业务专项资助项目 |
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| 摘 要： | 从海南某农药厂地土壤中分离到1株能很好降解毒死蜱的菌株WJI-063,经形态特征、生理生化及16s rDNA序列分析,将该菌株初步鉴定为蜡状芽孢杆菌(Bacillus cereus).降解酶降解性能研究表明,该菌株能以毒死蜱作为唯一碳源生长,并且对毒死蜱起主要降解作用的是胞内酶,其传代降解代谢活性稳定.以牛血清白蛋白为标准蛋白,测得粗提酶中可溶性蛋白含量为0.044 mg/mL;在pH5.0～8.0范围内,酶活力表现稳定,通过双曲线法认为该酶降解毒死蜱的最适pH值为8.0;毒死蜱降解酶热稳定性差,最适温度为25℃;该酶与毒死蜱底物结合力强,对毒死蜱具有较好的降解效果,米氏常数Km为201.92nmol/L,最大降解速率为399.36nmol/mg·min.
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| 关 键 词： | 毒死蜱 生物降解 酶促降解 蜡状芽孢杆菌 |
Identification of a Chlorpyrifos-degrading Bacterial Strain and Characterization of Its Enzymatic Degradation |
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| Wang Li,Yu Xianmei,He Chunping and Zheng Fucong.Identification of a Chlorpyrifos-degrading Bacterial Strain and Characterization of Its Enzymatic Degradation[J].Chinese Journal of Tropical Crops,2009,30(3):357-361. |
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| Authors: | Wang Li Yu Xianmei He Chunping and Zheng Fucong |
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| Institution: | Wang Li1,2,Yu Xianmei2,He Chunping2,Zheng Fucong1,2 1 College of Environment , Plant Protection,Hainan University,Danzhou,Hainan 571737,2 Environment , Plant Protection Institute,CATAS |
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| Abstract: | A strain of chlorpyrifos -degrading microorganism named WJI -063 was isolated from the soil polluted by the pesticide chlorpyrifos for a long term. According to its morphology and biochemical properties and 16S rDNA sequence analysis, the strain was identified as Bacillus cereus. The degradation of degrading enzyme showed that chlorpyrifos could be used as the only carbon source for the strain growth and that the intracellular enzyme played a major role in the degradation of chlorpyrifos and had a stable de... |
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| Keywords: | chlorpyrifos biodegradation enzymatic degradation Bacillus cereus |
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