| 中蜂与意蜂王浆中DNA的RAPD分析 |
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| 引用本文： | 邹阳,黄康,颜伟玉,曾志将.中蜂与意蜂王浆中DNA的RAPD分析[J].江西农业大学学报,2007,29(4):631-633. |
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| 作者姓名： | 邹阳 黄康 颜伟玉 曾志将 |
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| 作者单位： | 江西农业大学,蜜蜂研究所,江西,南昌,330045 |
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| 基金项目： | 江西省自然科学基金资助项目(0530031) |
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| 摘 要： | 以中华蜜蜂(Apis cerana cerana)和意大利蜜蜂(Apis mellifera ligustica)王浆为材料,用改进的苯酚-氯仿法提取蜂王浆中的DNA,利用35个随机引物对提取出来的DNA进行RAPD扩增,结果表明:有6个随机引物能扩增出谱带,其中1个引物对所有王浆DNA样品的扩增结果完全相同,5个引物出现多态性。共检测到32条扩增谱带,其中24条为多态带。
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| 关 键 词： | 蜜蜂 王浆 DNA提取 RAPD |
| 文章编号： | 1000-2286（2007）04-0631-03 |
| 收稿时间： | 2006-12-22 |
| 修稿时间： | 2006-12-22 |
The Assessment of DNA in Royal Jelly from Apis mellifera and Apis cerana by RAPD markers |
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| ZOU Yang,HUANG Kang,YAN Wei-yu,ZENG Zhi-jiang.The Assessment of DNA in Royal Jelly from Apis mellifera and Apis cerana by RAPD markers[J].Acta Agriculturae Universitis Jiangxiensis,2007,29(4):631-633. |
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| Authors: | ZOU Yang HUANG Kang YAN Wei-yu ZENG Zhi-jiang |
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| Institution: | Honeybee Research Institute, JAU, Nanchang 330045, China |
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| Abstract: | The experiment used royal jelly of Apis cerana and Apis mellifera as materials,extracted the DNA of royal jelly with modified phenol-chloroform method,the extracted DNA was analyzed by RAPD with 35 arbitrary primers.The results showed that: in the 35 primers that were tested,1 obtained the same bands.5 were polymorphous,a total of 32 bands were obtained,of which 24 were polymorphic. |
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| Keywords: | honeybee royal jelly DNA extraction RAPD |
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