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Effect of ascorbic acid supplement in vitro onrainbow trout sperm viability |
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| Authors: | A Ciereszko K Dabrowski |
| Institution: | (1) School of Natural Resources, The Ohio State University, Columbus, OH, 43210, USA;;(2) Department of Molecular Andrology, Institute of Animal Reproduction andFood Research, Polish Academy of Sciences, 10-718 Olsztyn, Poland;(3) School of Natural Resources, The Ohio State University, Columbus, OH 43210, USA |
| Abstract: | We evaluated motility and fertilizing ability of rainbow trout Oncorhynchus mykiss semen obtained from fish fed diets without ascorbic acid and a diet supplemented with 870 mg kgminus 1 of ascorbyl monophosphate. Semen was stored in vitro on ice (0 °C) during 14 days. The spermatozoa from the supplemented group had the highest motility and lowest decline in fertilizing ability after storage. Lack of a positive effect of exogenous vitamin C on semen in fish deficient in ascorbic acid (milt was supplemented with 50 mg l–1 of ascorbic acid) suggests that the positive effect of ascorbic acid on semen quality is related to its long-term effects during spermatogenesis. |
| Keywords: | ascorbic acid rainbow trout (Oncorhynchus mykiss) semen sperm viability |
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