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甜瓜重组自交系群体SSR遗传图谱构建及纯雌性基因定位
引用本文:高美玲,朱子成,高鹏,栾非时.甜瓜重组自交系群体SSR遗传图谱构建及纯雌性基因定位[J].园艺学报,2011,38(7):1308-1316.
作者姓名:高美玲  朱子成  高鹏  栾非时
作者单位:(1东北农业大学园艺学院,哈尔滨150030;2齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006)
基金项目:国家自然科学基金项目(30871723,31071812); 国家西瓜甜瓜产业技术体系分子育种岗位项目(CARS-26-02); 农业部‘948’项目(2011-S17); 黑龙江省普通高等学校青年学术骨干支持计划项目(1155G65); 黑龙江省高校重点实验室开放基金项目(GS2009007); 齐齐哈尔大学青年教师科研启动项目(2010K-Z04)
摘    要: 以甜瓜(Cucumis melo L.)纯雌株厚皮甜瓜品系WI998为母本,雌雄异花同株薄皮甜瓜品系3-2-2为父本杂交,通过单粒传得到了含有185个F6︰7家系的重组自交系分离群体,构建SSR分子遗传图谱。1 219对SSR引物在亲本间有多态性的为215对,多态率17.6%,构建的图谱共包含210个SSR标记,分属18个连锁群,覆盖基因组长度为937.1 cM,标记间平均距离为4.4 cM。将与性别表达密切相关的纯雌性基因(g)定位到了第1连锁群上,其两侧标记为NR3和MU8294_1,与g基因的遗传间距分别为1.2 cM和2.6 cM。

关 键 词:甜瓜  重组自交系群体  SSR  遗传图谱  纯雌性  基因定位
收稿时间:2011-03-30

A Microsatellite-based Genetic Map of Melon and Localization of Gene for Gynoecious Sex Expression Using Recombinant Inbred Lines
GAO Mei-ling,ZHU Zi-cheng,GAO Peng, LUAN Fei-shi.A Microsatellite-based Genetic Map of Melon and Localization of Gene for Gynoecious Sex Expression Using Recombinant Inbred Lines[J].Acta Horticulturae Sinica,2011,38(7):1308-1316.
Authors:GAO Mei-ling    ZHU Zi-cheng  GAO Peng  LUAN Fei-shi
Institution:(1 College of Horticulture,Northeast Agricultural University,Harbin 150030,China;2College of Life Science,Agriculture and Forestry,Qiqihaer University,Qiqihaer,Heilongjiang 161006,China)
Abstract:A recombinant inbred line(RIL)mapping population of melon,Cucumis melo L. comprising of 185 F6︰7 families was developed from a cross between the monoecious inbred line 3-2-2(AAGG)and the gynoecious inbred line WI998(AAgg)through single-seed descent(SSD). This RIL population was used to develop a linkage map with microsatellite(or simple sequence repeat,SSR)markers. Of 1 219 SSR markers screened,215(17.6%)were polymorphic between the two parental lines which were applied for linkage analysis in 185 RILs. The resulting genetic map consisted of 18 linkage groups spanning 937.1 cM with a mean marker interval of 4.4 cM. Two SSR markers,NR3 and MU8294_1 were identified flanking the g locus in linkage group 1 which was 1.2 cM and 2.6 cM away from the gynoecy gene,respectively.
Keywords:melon  Cucumis melo L    RIL  SSR  genetic map  gynoecious  localization of gene  
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