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东北百里香组培再生体系的建立
引用本文:王玲,杨丽鹏,张秀珍,马喜娟.东北百里香组培再生体系的建立[J].园艺学报,2011,38(6):1185-1190.
作者姓名:王玲  杨丽鹏  张秀珍  马喜娟
作者单位:(东北林业大学园林学院,哈尔滨150040)
基金项目:国家自然科学基金项目(30872062); 中央高校基本科研业务费专项(DL09CA10); 黑龙江省自然科学基金项目(C200811); 哈尔滨市科技攻关项目(2008AA6CN090); 东北林业大学研究生科技创新项目
摘    要: 以中国特有地被植物东北百里香为试材,研究了植物生长调节剂组合对腋芽萌发和茎段、叶片外植体愈伤诱导和不定芽分化的影响。结果表明:百里香茎段腋芽可直接诱导萌发,在MS + 6-BA 0.5 mg · L-1 + NAA 0.1 mg · L-1培养基上萌发率最高,达74%,在MS + 6-BA 0.5 mg · L-1 + NAA 0.1 mg · L-1的培养基上增殖倍数为36.43。由茎段萌发的组培苗在1/2MS + IBA 0.5 mg · L-1的培养基中20 d后生根率100%,移栽成活率76.7%。继代培养中叶片外植体可以诱导出愈伤组织,但是不能进一步分化成苗。茎段愈伤诱导的最适培养基为MS + 6-BA 0.5 mg · L-1 + 2,4-D 1.0 mg · L-1,再分化培养基为MS + 6-BA(0.1 ~ 1.0)mg · L-1 + GA3(0.1 ~ 0.5)mg · L-1,分化率为33.3%。

关 键 词:东北百里香  再生体系  植物生长调节剂
收稿时间:2011-04-06

The Establishment of the Adventitious Bud Regeneration System of Thymus mandschuricus
WANG Ling,YANG Li-peng,ZHANG Xiu-zhen,Ma Xi-juan.The Establishment of the Adventitious Bud Regeneration System of Thymus mandschuricus[J].Acta Horticulturae Sinica,2011,38(6):1185-1190.
Authors:WANG Ling  YANG Li-peng  ZHANG Xiu-zhen  Ma Xi-juan
Institution:(The College of Landscape Architecture,Northeast Forestry University,Harbin 150040,China)
Abstract:Understand the effect of different combinations of plant growth regulators on bud germination,callus growth induction of stem and leaves,and the regeneration of adventitious buds,by using different tissues of Thymus mandschuricus Ronn.,an endemic ground-cover species of China,as explants. The research results indicated that the axillary buds of Th. mandschuricus can directly germinate in MS + 6-BA 0.5 mg · L-1 + NAA 0.1 mg · L-1 and the germination rate was 74%. The proliferation of axillary buds on MS medium containing 0.5 mg · L-1 6-BA and 0.01 mg · L-1 NAA was achieved to 36.43. The best medium for rooting was 1/2MS + IBA 0.5 mg · L-1. The rooting ratio was 100%. The survival rate reaches 76.7%. Callus can be inducted through the leaf in subculture but can not develop further. The optimizing medium for pedicels explants inducing callus is MS + 6-BA 0.5 mg · L-1 + 2,4-D 1.0 mg · L-1 and inducing multiple buds is MS + 6-BA(0.1–1.0)mg · L-1 + GA3(0.1–0.5)mg · L-1 with the buds induction frequency of 33.3%.
Keywords:Thymus mandschuricus  adventitious bud regeneration system  plant growth regulator  
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