| 藜草花叶病毒外壳蛋白基因的克隆与RT-PCR检测 |
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| 引用本文： | 陈青,李桂芬,董菁,陈红运,廖富荣,陈洪俊,朱水芳.藜草花叶病毒外壳蛋白基因的克隆与RT-PCR检测[J].植物病理学报,2010,40(4):438-441. |
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| 作者姓名： | 陈青 李桂芬 董菁 陈红运 廖富荣 陈洪俊 朱水芳 |
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| 作者单位： | 1 厦门出入境检验检疫局, 厦门 361026;2 中国检验检疫科学研究院, 北京 100029 |
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| 基金项目： | 国家科技支撑计划项目，公益性行业科研专项 |
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| 摘 要： | The nucleotide sequence of coat protein (cp)gene of Sowbane mosaic virus (SoMV)was determined. The cp gene of SoMV consists of 726 nucleotides and encodes a putative protein of 241 amino acid re-sidues. Sequence comparison showed that SoMV was most closely related to Rubus chlorotic mottle virus compared to other sobemoviruses. A primer pair was designed for the detection of SoMV based upon the determined cp nucleotide sequence. An expected fragment with 510 bp could be obtained from SoMV, while no specific band was observed from the healthy control. The result showed that the RT-PCR assay with the primer pair was suitable for the specific detection of SoMV.
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Cloning of coat protein gene and RT-PCR detection of Sowbane mosaic virus |
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| CHEN Qing,LI Gui-fen,DONG Jing,CHEN Hong-yun,LIAO Fu-rong,CHEN Hong-jun,ZHU Shui-fang.Cloning of coat protein gene and RT-PCR detection of Sowbane mosaic virus[J].Acta Phytopathologica Sinica,2010,40(4):438-441. |
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| Authors: | CHEN Qing LI Gui-fen DONG Jing CHEN Hong-yun LIAO Fu-rong CHEN Hong-jun ZHU Shui-fang |
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| Institution: | 1 Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361026, China;2 Chinese Academy of Inspection and Quarantine, Beijing 100029, China |
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| Abstract: | The nucleotide sequence of coat protein (cp)gene of Sowbane mosaic virus (SoMV)was determined. The cp gene of SoMV consists of 726 nucleotides and encodes a putative protein of 241 amino acid re-sidues. Sequence comparison showed that SoMV was most closely related to Rubus chlorotic mottle virus compared to other sobemoviruses. A primer pair was designed for the detection of SoMV based upon the determined cp nucleotide sequence. An expected fragment with 510 bp could be obtained from SoMV, while no specific band was observed from the healthy control. The result showed that the RT-PCR assay with the primer pair was suitable for the specific detection of SoMV. |
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| Keywords: | Sowbane mosaic virus coat protein detection |
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