AFLP analysis of genetic diversity in populations of <Emphasis Type="Italic">Botrytis elliptica</Emphasis> and <Emphasis Type="Italic">Botrytis tulipae</Emphasis> from the Netherlands |
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| Authors: | M Staats P van Baarlen J A L van Kan |
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| Institution: | (1) Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands;(2) Present address: ENZA Seeds, Enkhuizen, The Netherlands;(3) Present address: Centre for Molecular and Biomolecular Informatics, Radboud University, Nijmegen, The Netherlands |
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| Abstract: | The objective of this study was to assess the genetic diversity and to infer the mode of reproduction of Botrytis
elliptica and B. tulipae in the Netherlands. First, three molecular typing methods were compared for their ability to differentiate isolates of B. tulipae, B. elliptica, and B. cinerea. The methods compared were multilocus sequencing, restriction analysis of the ribosomal intergenic spacer (IGS) region, and
amplified fragment length polymorphism (AFLP) analysis. AFLP fingerprinting provided the most efficient method to differentiate
isolates within each Botrytis species and therefore this method was used for population analyses of B. elliptica and B. tulipae. Isolates of both species were sampled during successive growing seasons in experimental field plots in Lisse and other locations
in the Netherlands. Among 174 B. elliptica isolates, 105 genotypes could be discriminated and 87 genotypes were found only once, reflecting high genotypic variation.
Clonal genotypes were found only within growing seasons and in one location. Linkage disequilibrium analyses indicated that
between 9.4% and 19.3% of the loci in clone-corrected samples were linked. The multilocus association index provided no evidence
for random mating. We conclude that sexual recombination occurs in the B. elliptica population. Among the 170 B. tulipae isolates, 25 genotypes could be discriminated and four genotypes were found only once, reflecting a low genotypic variation.
Clonal genotypes were frequently found in different growing seasons and different locations. Linkage disequilibrium analyses
indicated that between 25.2% and 48.6% of the loci in clone-corrected samples were linked. We conclude that the B. tulipae population is mainly clonal with some recombination. |
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| Keywords: | Botrytis AFLP Genotypes Clonal diversity Recombination |
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