| 基于SSR标记的云南腾冲水稻的遗传多样性分析 |
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| 引用本文： | 李松,张世成,董云武,施德林,史云东.基于SSR标记的云南腾冲水稻的遗传多样性分析[J].作物杂志,2019,35(5):15-30. |
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| 作者姓名： | 李松 张世成 董云武 施德林 史云东 |
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| 作者单位： | 1 玉溪师范学院化学生物与环境学院,653100,云南玉溪2 玉溪市种子管理站,653100,云南玉溪 |
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| 基金项目： | 云南省地方本科高校联合青年项目(2017FH001-099) |
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| 摘 要： | 利用中国农业行业标准（NY/T 1433-2014）推荐的SSR核心引物分析腾冲本地糯谷和推广种植的多个品种间的遗传多样性。结果表明：18对SSR引物在20个品种间具有多态性,共扩增出了82个多态性片段,平均每对引物检测到4.6个多态性片段,变幅为2~8个;Nei′s多样性指数为0.155~0.384,平均为0.248;Shannon′s信息指数为0.280~0.567,平均为0.398;SSR多态性指数（PIC）平均值为0.63,变幅为0.26~0.84。遗传相似系数平均0.710,变幅为0.410~0.976。聚类分析结果显示,在遗传相似系数0.70处可将20个品种划分为4类,其中第Ⅰ类和第Ⅱ类分别包括4和5个品种,腾冲糯谷为第Ⅲ类,第Ⅳ类包括10个品种。研究结果表明腾冲糯谷与推广种植水稻品种间遗传基础丰富,多态性好,为腾冲市水稻种质改良和新品种选育提供了参考依据。
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| 关 键 词： | 水稻 SSR分子标记 种质资源 遗传多样性 |
| 收稿时间： | 2019-05-06 |
Genetic Diversity Analysis of Rice Varieties in Tengchong,Yunnan Based on SSR Markers |
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| Li Song,Zhang Shicheng,Dong Yunwu,Shi Delin,Shi Yundong.Genetic Diversity Analysis of Rice Varieties in Tengchong,Yunnan Based on SSR Markers[J].Crops,2019,35(5):15-30. |
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| Authors: | Li Song Zhang Shicheng Dong Yunwu Shi Delin Shi Yundong |
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| Institution: | 1 School of Chemical Biology and Environment, Yuxi Normal University, Yuxi 653100, Yunnan, China2 Yuxi Seed Management Station, Yuxi 653100, Yunnan, China |
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| Abstract: | The genetic diversity analysis among several of indigenous waxy rice and it’s popularization grown in Tengchong were carried out based on SSR primers recommended by Chinese Agricultural Industry Standard (NY/T 1433-2014). The results showed that 18 pairs of SSR primers presented polymorphic among 20 varieties, and 82 polymorphic fragments were detected. The number of polymorphic fragments detected by each pair of primers was 2 to 8, with an average of 4.6. The Nei′s diversity index was 0.155 to 0.384, with an average of 0.284. The Shannon′s information index was 0.280 to 0.567, with an average of 0.398. The SSR polymorphism information content (PIC) distribution range from 0.26 to 0.84, with an average of 0.63. The genetic similarity coefficient range from 0.410 to 0.976, with an average of 0.710. The cluster analysis showed that when the genetic similarity coefficient was 0.70, 20 varieties were divided into 4 groups, of which groupsⅠand groupsⅡincluded 4 and 5 varieties, respectively, of which groupsⅢonly included indigenous waxy valley while the groupⅣincluded 10 varieties. The results showed that Tengchong waxy rice and extended varieties had rich genetic diversity and good polymorphism, which provided a reference for rice germplasm improvement and new variety breeding in Tengchong. |
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| Keywords: | Rice SSR markers Germplasm resources Genetic diversity |
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