Relationships among some cornelian cherry genotypes (<Emphasis Type="Italic">Cornus mas</Emphasis> L.) based on RAPD analysis |
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| Authors: | Sezai Ercisli Emine Orhan Ahmet Esitken Nalan Yildirim Guleray Agar |
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| Institution: | (1) Department of Horticulture, Faculty of Agriculture, Ataturk University, Erzurum, 25240, Turkey;(2) Department of Biology, Faculty of Art and Science, Ataturk University, Erzurum, 25240, Turkey |
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| Abstract: | Turkey is an important producer of cornelian cherries (Cornus mas L.), especially in northern Anatolia. Seed propagation and long-term human selection has given rise to a great diversity
of trees. Twenty-six cornelian cherry genotypes (CC1–CC26) from the Coruh Valley in northern Anatolia were evaluated for genetic
relationships by using Randomly Amplified Polymorphic DNA (RAPD) markers, based on 56 decamer random primers, seven of which
showed reliable polymorphisms. These seven primers generated 80 markers, with 77 (96.25%) displaying polymorphisms. Cluster
analysis of the cornelian cherry genotypes was performed based on data from polymorphic RAPD bands, by using Jaccard’s similarity
coefficient and the Unweighted pair-group method with arithmetic average (UPGMA) clustering method. A similarity matrix showed
that the highest genetic similarity (0.913) was between CC15 and CC16 and the least (0.129) was between CC4 and CC16. The
cophenetic correlation coefficient between the similarity matrix and the cophenetic matrix of the dendrogram was relatively
high (r = 0.87), supporting the validity of the dendrogram. Based on these results, RAPD analysis can be used for the characterization
and grouping of cornelian cherry genotypes. Genetically divergent genotypes identified in this study may be useful for future
breeding programs. This is the first study demonstrating that RAPD analyses can be used to differentiate and classify cornelian
cherry genotypes. |
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| Keywords: | Cornus DNA marker Genetic diversity |
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