Genetic diversity in wild barley (Hordeum spontaneum C. Koch) in the Near East: a molecular analysis using Random Amplified Polymorphic DNA (RAPD) markers |
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| Authors: | Bernard R Baum E Nevo Douglas A Johnson A Beiles |
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| Institution: | (1) Centre for Land and Biological Resources Research, Agriculture and Agri-Food Canada, Central Experimental Farm, Ottawa, Ontario, Canada, K1A 0C6;(2) Institute of Evolution, University of Haifa, Mt. Carmel, Haifa, 31905, Israel;(3) Ottawa-Carleton Institute of Biology, University of Ottawa, 30 Marie Curie, Ottawa, Ontario, Canada, K1N 6N5 |
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| Abstract: | We analyzed the genetic diversity in 88 genotypes from 20 populations of wild barley (Hordeum spontaneum C. Koch) from Israel, Turkey and Iran, by randomly amplified polymorphic DNA (RAPD). Twenty two of the 33 primers used yielded scorable products with 1–11 polymorphic bands. No duplicate patterns were found except for four haplotypes.When the total genetic diversity was estimated, 75% of the variation detected was partitioned within the 88 genotypes and 25% among the populations. When variation between countries was assessed, no substantial differences were found, because most of the variation detected (97%) was partioned within the 20 populations and the remainder among countries. The results of this limited survey indicate that the extensive genetic diversity is present in natural stands of wild barley throughout the Fertile Crescent. |
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| Keywords: | Genetic polymorphism wild barley Hordeum spontaneum |
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