| 基于线粒体D-loop区与COI基因序列比较分析养殖与野生银鲳群体遗传多样性 |
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| 引用本文： | 彭士明,施兆鸿,侯俊利.基于线粒体D-loop区与COI基因序列比较分析养殖与野生银鲳群体遗传多样性[J].水产学报,2010,34(1):19-25. |
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| 作者姓名： | 彭士明 施兆鸿 侯俊利 |
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| 作者单位： | 中国水产科学研究院东海水产研究所,上海,200090 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费(东2008M14,东2007Z02);沪农科攻字(2007)第4-1号 |
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| 摘 要： | 运用线粒体D-loop区与COI基因片段序列比较分析了养殖与野生银鲳群体的遗传多样性。研究结果显示,线粒体D-loop区片段中,A、T、C与G4种核苷酸的平均含量分别为40.00%、30.55%、16.75%和12.70%,A+T的含量为70.55%,明显高于G+C的含量。COI基因片段中,A、T、C和G的平均含量分别为25.85%、33.90%、21.30%和18.85%,A+T的含量(59.75%)同样高于G+C的含量。基于D-loop序列分析所得出的两群体总的变异位点、单倍型数、单倍型多样性、核苷酸多样性及平均核苷酸差异数分别为19、15、0.895、0.007和2.505。基于COI基因所得出的两群体总的变异位点、单倍型数、单倍型多样性、核苷酸多样性及平均核苷酸差异数分别为33、17、0.713、0.004和2.239。基于线粒体D-loop区与COI基因片段序列的研究结果均显示,养殖银鲳群体的遗传多样性低于野生群体的遗传多样性。养殖群体基于线粒体D-loop区与COI基因片段分析得出的单倍型多样性分别为0.562与0.571,野生群体基于线粒体D-loop区与COI基因片段分析得出的单倍型多样性分别...
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| 关 键 词： | 银鲳 线粒体DNA 养殖 野生 遗传多样性 |
| 收稿时间： | 2009/3/20 0:00:00 |
| 修稿时间： | 6/2/2009 12:00:00 AM |
Comparative analysis on the genetic diversity of cultured and wild silver pomfret populations based on mtD-loop and COI gene |
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| PENG Shi-ming,SHI Zhao-hong,HOU Jun-li.Comparative analysis on the genetic diversity of cultured and wild silver pomfret populations based on mtD-loop and COI gene[J].Journal of Fisheries of China,2010,34(1):19-25. |
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| Authors: | PENG Shi-ming SHI Zhao-hong HOU Jun-li |
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| Institution: | PENG Shi-ming,SHI Zhao-hong,HOU Jun-li (East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China) |
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| Abstract: | The genetic diversity of cultured and wild silver pomfret (Pampus argenteus) populations was analyzed based on mtD-loop and COI gene. The results showed that the average A,T,C and G contents in D-loop were 40.00%,30.55%,16.75% and 12.70%,respectively,and the contents of A + T were 70.55%,higher than those of G + C. In the COI gene,the average A,T,C and G contents in D-loop gene were 25.85%,33.90%,21.30% and 18.85%,respectively,and the contents of A + T were 59.75%,also higher than those of G + C. The total ... |
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| Keywords: | silver pomfret mtDNA culture wild genetic diversity |
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