| dapA基因缺失对大肠杆菌L-苏氨酸产量的影响 |
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| 引用本文： | 张力,李伟星,杨晓志,张君胜.dapA基因缺失对大肠杆菌L-苏氨酸产量的影响[J].甘肃农业大学学报,2011(4):151-156. |
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| 作者姓名： | 张力 李伟星 杨晓志 张君胜 |
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| 作者单位： | 江苏畜牧兽医职业技术学院; |
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| 基金项目： | 江苏畜牧兽医职业技术学院院长基金(200502) |
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| 摘 要： | 采用Red重组技术,将大肠杆菌K12的dapA基因置换,构建dapA基因缺失的突变株,通过发酵测定突变株L-苏氨酸的产量.获得的dapA基因缺失突变株命名为K12△dapA.结果表明:在相同培养条件下发酵48 h,重组菌株K12△dapA发酵液中积累的L-苏氨酸达3.71 g/L,是Ecoli K12原始菌株的3倍.d...
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| 关 键 词： | L-苏氨酸 大肠杆菌 dapA基因 Red重组 |
Effect of dapA gene deletion on L-threonine production by Escherichia coli |
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| ZHANG Li,LI Wei-xing,YANG Xiao-zhi,ZHANG Jun-sheng.Effect of dapA gene deletion on L-threonine production by Escherichia coli[J].Journal of Gansu Agricultural University,2011(4):151-156. |
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| Authors: | ZHANG Li LI Wei-xing YANG Xiao-zhi ZHANG Jun-sheng |
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| Institution: | ZHANG Li,LI Wei-xing,YANG Xiao-zhi,ZHANG Jun-sheng(Jiangsu Profession and Technical College of Animal Science and Veterinary Medicine,Taizhou 225300,China) |
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| Abstract: | Using Red recombination technique,the dapA gene in Escherichia coli 'K12'was replaced,then a mutant without dapA gene was gained,and it was named as K12△dapA.Thus the K12△dapA and Escherichia coli 'K12'were both fermentated under same condition for 48 h,the L-threonine content in K12△dapA broth was 3.71 g/L,which was 3 times of that in Escherichia coli 'K12'broth.The results indicated that dapA gene deletion improved the accumulation of L-threonine. |
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| Keywords: | L-threonine Escherichia coli dapA gene Red recombination |
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