| 测定猪肉中土霉素及金霉素残留的HPLC法建立 |
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| 引用本文： | 智若岚,刘中成,李广兴,张瑞莉.测定猪肉中土霉素及金霉素残留的HPLC法建立[J].东北农业大学学报,2011,42(9):141-144. |
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| 作者姓名： | 智若岚 刘中成 李广兴 张瑞莉 |
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| 作者单位： | 1. 东北农业大学动物医学学院,哈尔滨,150030
2. 哈尔滨市鲁药饲料监察所,哈尔滨,150040 |
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| 基金项目： | 黑龙江省青年基金项目(QC08C96); 黑龙江省教育厅科研项目(10531005) |
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| 摘 要： | 文章建立了测定猪肉组织中土霉素及金霉素残留量的HPLC法.该方法按照国家检测标准采用了5％高氯酸50 mL为样品提取液；乙腈+0.01 mol·L-1磷酸二氢钠溶液(用30％硝酸调节pH2.5)=35:65为流动相；检测波长为355nm,进行测定.在此色谱条件下,土霉素、金霉素标准品浓度在0.1～1.0 μg· mL-...
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| 关 键 词： | 高效液相色谱法 土霉素 金霉素 猪肉 残留检测 |
Determination of residues of oxytetracycline and chlortetracycline in pork by HPLC |
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| ZHI Ruolan,LIU Zhongcheng,LI Guangxing,ZHANG Ruili.Determination of residues of oxytetracycline and chlortetracycline in pork by HPLC[J].Journal of Northeast Agricultural University,2011,42(9):141-144. |
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| Authors: | ZHI Ruolan LIU Zhongcheng LI Guangxing ZHANG Ruili |
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| Institution: | ZHI Ruolan1,LIU Zhongcheng2,LI Guangxing1,ZHANG Ruili1(1. College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China,2.Harbin Institute of Veterinary Drug and Feed Control,Harbin 150040,China) |
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| Abstract: | To establish an HPLC method for determination of oxytetracycline and chlorotetracycline in pork.According to the national detective standard,5% perchloric acid(50 mL) was adopted as sample extract,acetonitrile+0.01 mol.L-1 sodium dihydrogen phosphate as mobile phase and 355 nm as detection wavelength.In this condition,the result showed that the standard substances concentration of oxytetracycline and chlorotetracycline linear correlation good to extent 0.1-1.0 μg.mL-1 and 0.2-2.0 μg.mL-1 on this chromatographic column condition,oxytetracycline: different concentration average recoveries were feom 78.06% to 87.21%,and RSD was 3.02%-7.72%.Chloroteracycline: different concentra-tion average recoveries was 76.45%-85.62% and RSD was 3.46%-6.32%.The lowest detectable limits of oxytetracycline and chlorotetracycline were 20 and10 μg.kg-1.The results indicated that the method presented was of convenience,simpleness,quickness and high sensitivity.And the method was applicable to proof quantitative analysis of oxytetracycline and chlorotetracycline residue. |
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| Keywords: | HPLC oxytetracycline chlorotetracycline pork residue detection |
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