cDNA cloning of Japanese oyster stress protein homologous to the mammalian 78-kDa glucose regulated protein and its induction by heatshock |
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| Authors: | Yoshihiro Yokoyama Hisashi Hashimoto Satoshi Kubota Akira Kuriyama Yoichi Ogura Shoshi Mizuta Reiji Yoshinaka Haruhiko Toyohara |
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| Institution: | Department of Marine Bioscience, Faculty of Biotechnology, Fukui Prefectural University, Obama, Fukui 917-0003,;Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601,;Division of Human Health and Medical Science, Graduate School of Kuroshio Science, Kochi University, Nankoku 783-8502,;Department of Life Science and Engineering, School of Science and Engineering, Tokyo Denki University, Hiki-gun, Saitama, 350-0394, and;Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan |
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| Abstract: | ABSTRACT: The 78-kDa glucose regulated protein (GRP78), a member of stress proteins, was cloned from a cDNA library of Japanese oyster Crassostrea gigas . The analysis on Japanese oyster GRP78 clone of approximately 2.6 kb revealed that the entire open reading frame was 1983 bp long and encoded 661 amino acid residues. At the DNA sequence level, the coding region of Japanese oyster GRP78 gene was 72, 62, and 62% identical to those of chicken GRP78, Japanese flounder HSP70, and Japanese flounder HSC71 genes, respectively. Deduced amino acid sequence of Japanese oyster GRP78 was 84, 62, and 62% identical to those of chicken GRP78, Japanese flounder HSP70, and Japanese flounder HSC71, respectively. Japanese oyster GRP78 contained an 18-residue sequence at the N-terminus that exhibits characteristics of a cleavable signal sequence. It also contained an ATPase domain, and a peptide-binding domain in addition to a Lys-Asp-Glu-Leu (KDEL) peptide motif that is involved in determining endoplasmic reticulum localization. Northern blot analysis showed that GRP78 mRNA was induced with heatshock treatment in the oyster tissues. |
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| Keywords: | Crassostrea gigas GRP78 heatshock Japanese oyster |
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