| 农杆菌介导法将FPFJ基因导入油菜的研究初报 |
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| 引用本文： | 黄琼华,杨光伟,李德谋,罗小英,裴炎.农杆菌介导法将FPFJ基因导入油菜的研究初报[J].西南农业大学学报,2002,24(2):124-127. |
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| 作者姓名： | 黄琼华 杨光伟 李德谋 罗小英 裴炎 |
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| 作者单位： | 西南农业大学生物技术中心 重庆400716
(黄琼华，杨光伟，李德谋，罗小英)，西南农业大学生物技术中心 重庆400716(裴炎) |
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| 摘 要： | 将拟南芥早花基因FPF1（flowering promoting factor 1)插入双元高效表达载体pBI 121中，构建了植物表达载体pBIUbi-FPF1。以“双低”甘蓝型晚熟油菜品种2000 F4102，2000 F4153的下胚轴为转化受体，通过农杆菌介导的遗传转化获得抗卡那霉素的再生转基因植株，并探讨了影响油菜再生频率以及遗传转化效率的几个因素。对部分经卡那霉素筛选得到的再生植株进行了PCR检测，其中70％以上的卡那霉素抗性植株表现阳性，初步证明FPF1基因已整合到油菜细胞核基因组中。
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| 关 键 词： | FPF1基因 转基因 油菜 农杆菌介导法 早熟性 卡那霉素 遗传转化 |
| 文章编号： | 1000-2642(2002)02-0124-04 |
| 修稿时间： | 2001年10月8日 |
PRELIMINARY STUDIES ON TRANSGENIC RAPESEED ( BRASSICA NAPUS L. ) WITH FPF1 GENE MDIATED BY AGROBACTERIUM TUMEFACIEN |
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| HUANG Qiong-hua,YANG Guang-wei,LI De-mou,LUO Xiao-ying,PEI Yan.PRELIMINARY STUDIES ON TRANSGENIC RAPESEED ( BRASSICA NAPUS L. ) WITH FPF1 GENE MDIATED BY AGROBACTERIUM TUMEFACIEN[J].Journal of Southwest Agricultural University,2002,24(2):124-127. |
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| Authors: | HUANG Qiong-hua YANG Guang-wei LI De-mou LUO Xiao-ying PEI Yan |
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| Abstract: | The plant expression vector pBIUbi- FPF1 was constructed by inserting Arabiopsis FPF1 (flowering promoting factor 1) gene into the plant high-efficient expression vector pBI 121. The hypocotyls segments from Brassica napus L. cultivars 2000 F4102 and 2000 F4153 were transformed by Agrobacterium -mediation method. Some kanamycin-resistant green shoots were obtained. Factors affecting differentiation, shoot regeneration and transformation frequency of oilseed rape hypocotyls were studied. Differentiation medium and AgNO 3 were shown to play important roles in green shoot formation in rape. Suitable pre-culture period, co-culture period, A. tumefaciens concentration and infecting period were key factors for transformation frequency. A preliminary molecular identification with the kan-resistant green shoots showed that 7 of the 10 green shoots amplified gave the expected fragment as the positive control in 698 bp, thus confirming successful integration of the foreign gene into the genomes of the oilseed rape cultivars. |
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| Keywords: | FPF1 gene transgenic oilseed rape Agrobacterium tumefaciens- mediated transformation early maturation |
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