A Set of SCAR Markers Efficiently Differentiating Hybrid Rice |
| |
| Authors: | LI Shu-jing XIE Hong-wei QIAN Ming-juan CHEN Guang-hui LI Shao-qing ZHU Ying-guo State |
| |
| Institution: | Key Laboratory of Hybrid Rice;Key Laboratory for Research and Utilization of Heterosis in Indica Rice,Ministry of Agriculture;Engineering Research Center for Plant Biotechology and Germplasm Utilization of Ministry of Education;College of Life Sciences,Wuhan University,Wuhan 430072,China;2Bangbu Medical College,Bangbu 233030,China;3College of Plant Science,Hunan Agricultural University,Changsha 410128,China) |
| |
| Abstract: | Molecular markers have been widely used in crop genetic improvement,seed test and genetic mapping.Of which,sequence characterized amplified region(SCAR) markers are particularly popular for its diversity,stable reproducibility,and suitability for analyzing large number of samples.In this study,500 random amplified polymorphic DNA(RAPD) primers were tested,and a set of SCAR markers comprising 37 pairs of loci-specific primers were developed from the DNA fragments ranging from 300 to 1000 bp which correspond to the stable,distinctive RAPD banding patterns.Using these SCAR markers,59 hybrid rice combinations were assessed and distinguished into 58 subgroups at the similarity coefficient of 0.97 in a genetic clustering tree based on the allele diversities of the SCAR markers.Furthermore,13 hybrid rice combinations were reassayed with 40 randomly selected simple sequence repeat(SSR) markers to evaluate the effectiveness of these SCAR markers.SSR markers produced similar results to SCAR markers as the 13 hybrid rice combinations were completely separated at the similarity coefficient of 0.91 in the clustering tree established from SSR patterns.Taken together,SCAR markers prove to be effective tools for identifying and differentiating hybrid rice combinations. |
| |
| Keywords: | hybrid rice molecular marker sequence characterized amplified region variety identification |
| 本文献已被 CNKI ScienceDirect 等数据库收录！ |
|
