Yield of embryos from sequential anther culture |
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| Authors: | L Y Shen R E Veilleux |
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| Institution: | 1. Department of Horticulture, Virginia Polytechnic Institute and State University, 24061-0327, Blacksburg, Virginia
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| Abstract: | Experiments were conducted on sequential anther culture,i.e., returning anthers into medium following the usual harvest of embryos six weeks after culture initiation. Three diploid interspecific hybrid potato clones were used. Additional embryos were produced after the first harvest, although the quality and regeneration frequency of such embryos were generally poorer than for first harvest embryos. In addition, embryos from the second harvest required more time to regenerate into plants. Significantly more embryos were produced when anthers were returned to the same medium (16 per flask) compared to fresh medium (4 per flask) in sequential anther culture. Regression analysis showed that embryo yields at the first and second harvests were significantly correlated (P<0.01). Poor anther culture yield at the first harvest was not caused by a longer lag period. Highly responsive cultures continued to yield embryos even 12 weeks after culture initiation. |
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